Phenotype Help

CIT2 / YCR005C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant displays decreased ability to utilize nonfermentable carbon sources such as glycerol, ethanol, and lactate; in large-scale studies the null mutant shows decreased thermotolerance, and sensitivity to a fragment of human Huntingtin; overexpression in a diploid confers increased pseudohyphal growth

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

14 entries for 14 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
chemical compound accumulation: abnormal
systematic mutation setnull
Allele: cit2-Δ
S288C alpha-amino acidDetails: Significantly altered free amino acid profile (X^2- test p < 0.01), showing 1 simultaneous amino acid change (Z-test, adjusted p < 0.01)
Mülleder M, et al. (2016) PMID:27693354
chemical compound accumulation: increased
systematic mutation setnull
Allele: cit2-Δ
S288C aspartate(1-)Mülleder M, et al. (2016) PMID:27693354
chronological lifespan: decreased
classical genetics null
Allele: cit2-Δ
S288CMedia: glucose, 2 %
Details: significant reduction in the CLS of the null mutant relative to wt under non-restricted conditions
Kwon YY, et al. (2024) PMID:38717792
competitive fitness: increased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: cit2-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 1.019
Breslow DK, et al. (2008) PMID:18622397
heat sensitivity: decreased
systematic mutation set

high throughput heat ramp cell death assay

null
Allele: cit2-Δ
S288CTemperature: elevated temperature
Details: ramp temperature from ambient to 30 deg C immediately, hold 1 min at 30 deg C, ramp temperature from 30 deg C to 62 deg C over 20 min, return to ambient
Teng X, et al. (2011) PMID:21814286
innate thermotolerance: decreased
systematic mutation setnull
Allele: cit2-Δ
S288CTreatment: heat shock, 50 °C
Details: acquired thermotolerance is unaffected
Gibney PA, et al. (2013) PMID:24167267
invasive growth: increased
homozygous diploid, systematic mutation setoverexpressionSigma1278bMedia: nitrogen-sufficient medium
Details: agar invasion score: 0.94; enhanced pseudohyphal growth, based on elevated invasiveness
Shively CA, et al. (2013) PMID:23410832
mitochondrial morphology: abnormal
classical genetics null
Allele: cit2-Δ
W303Details: percentage of cells with tubular mitochondria is reduced from ~63% in wt cells to 50% in the null mutant; further decreased to ~37% by overexpression of OLE1
Alsayyah C, et al. (2024) PMID:38669296
protein/peptide distribution: abnormal
Reporter: Fum1p
classical geneticsnull
Allele: cit2-Δ
S288CDetails: cytosolic activity and protein levels of fumarase are decreased, although total cellular fumarase activity is elevated
Regev-Rudzki N, et al. (2009) PMID:19415796
resistance to chemicals: decreased
heterozygous diploid, systematic mutation set null
Allele: cit2-Δ
S288C30 μg/mL gemfibrozilLum PY, et al. (2004) PMID:14718172
Showing 1 to 10 of 14 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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