Phenotype Help

YCR023C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
YCR023C null mutants are viable but show decreased competitive fitness in minimal and synthetic oak exudate media but increased competitive fitness in the presence of ethanol. They also display decreased heat sensitivity and increased resistance to metals, such as gadolinium and yttrium chloride. Additionally, these mutants have a decreased rate of nitrogen source utilization (specifically glutamate) and abnormal vacuolar morphology, with a small defect in vacuolar fragmentation in response to salt addition.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

9 entries for 8 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
competitive fitness: decreased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: ycr023c-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 0.991
Breslow DK, et al. (2008) PMID:18622397
competitive fitness: decreased
systematic mutation setnull
Allele: ycr023c-Δ
S288CMedia: synthetic oak exudate medium, OAK
Qian W, et al. (2012) PMID:23103169
competitive fitness: increased
systematic mutation setnull
Allele: ycr023c-Δ
S288C ethanolQian W, et al. (2012) PMID:23103169
heat sensitivity: decreased
systematic mutation set

high throughput heat ramp cell death assay

null
Allele: ycr023c-Δ
S288CTemperature: elevated temperature
Details: ramp temperature from ambient to 30 deg C immediately, hold 1 min at 30 deg C, ramp temperature from 30 deg C to 62 deg C over 20 min, return to ambient
Teng X, et al. (2011) PMID:21814286
metal resistance: increased
systematic mutation set null
Allele: ycr023c-Δ
S288C3.9 mM gadolinium(3+)Treatment: gadolinium (III) chloride hexahydrate, 3.9 mM
Details: medium resistance; confirmed by serial dilution spot assays on 4.0 mM Gd
Grosjean N, et al. (2023) PMID:37630673
resistance to chemicals: increased
systematic mutation set null
Allele: ycr023c-Δ
S288C3.75 mM yttrium chlorideGrosjean N, et al. (2018) PMID:30233513
utilization of nitrogen source: decreased rate
systematic mutation set

prototrophic version of yeast deletion collection

null
Allele: ycr023c-Δ
S288C glutamate(1-)VanderSluis B, et al. (2014) PMID:24721214
vacuolar morphology: abnormal
systematic mutation set

screen all non-essential genes for inability to fragment vacuoles in response to salt addition

null
Allele: ycr023c-Δ
S288C0.4 M sodium chlorideDetails: small defect in vacuolar fragmentation
Michaillat L and Mayer A (2013) PMID:23383298
viable
systematic mutation setnull
Allele: ycr023c-Δ
S288CGiaever G, et al. (2002) PMID:12140549
Showing 1 to 9 of 9 entries

Shared Phenotypes

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