SLM5 / YCR024C Overview
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Slm5p is 492 amino acids long, of average half-life, low in abundance; contains 4 aspartyl/asparaginyl-tRNA synthetase class IIb domains
- Length (a.a.)
- 492
- Mol. Weight (Da)
- 56791.5
- Isoelectric Point
- 8.64
- Median Abundance (molecules/cell)
- 1501 +/- 965
- Half-life (hr)
- 9.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SLM5 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Mitochondrial asparaginyl-tRNA synthetase that performs asparaginyl-tRNA aminoacylation by coupling asparagine to asparaginyl-tRNA
View computational annotations
Molecular Function
- Manually Curated
- enables asparagine-tRNA ligase activity (IDA)
Biological Process
- Manually Curated
- involved in asparaginyl-tRNA aminoacylation (IDA)
- involved in mitochondrial asparaginyl-tRNA aminoacylation (IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- SLM5/YCR024C is a non-essential gene; null mutants are viable but exhibit a range of phenotypic changes including abnormal or absent mitochondrial genome maintenance, abnormal mitochondrial morphology, decreased mitophagy, decreased acid pH resistance, decreased chronological lifespan, decreased competitive fitness, decreased desiccation resistance, increased heat sensitivity, increased metal resistance, decreased oxidative stress resistance, altered resistance to chemicals (both increased and decreased), absent or decreased respiratory growth, decreased toxin resistance, decreased utilization of various carbon sources, decreased UV resistance, and decreased vegetative growth rate.
Classical Genetics
- null
- acid pH resistance: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- haploinsufficient
- haploproficient
- heat sensitivity: increased
- metal resistance: increased
- mitochondrial genome maintenance: absent
- mitochondrial morphology: abnormal
- mitophagy: decreased
- oxidative stress resistance: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- respiratory growth: decreased rate
- toxin resistance: decreased
- utilization of carbon source: decreased
- UV resistance: decreased
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast SLM5 is homologous to human NARS2 and has been used to study mutations found in patients with mitochondrial disease presenting as developmental delay, epilepsy, and complex I deficiency
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Slm5p interacts physically with proteins involved in rRNA processing and ribosomal small subunit biogenesis; SLM5 interacts genetically with genes involved in transcription
183 total interactions for 180 unique genes
Physical Interactions
- Affinity Capture-MS: 29
- Affinity Capture-RNA: 3
- Biochemical Activity: 2
- Protein-peptide: 1
- Reconstituted Complex: 2
- Two-hybrid: 4
Genetic Interactions
- Dosage Growth Defect: 1
- Negative Genetic: 119
- Positive Genetic: 12
- Synthetic Growth Defect: 3
- Synthetic Lethality: 7
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2008-07-14
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.