SEC3 / YER008C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

SEC3/YER008C mutants exhibit temperature sensitivity (heat at 34–37°C; cold at 23°C) and reduced vegetative growth, with viability depending on strain/background (inviable in the S288C deletion set but viable in XJ24-24a; haploinsufficient as a heterozygote). Loss- and conditional-function alleles cause broad secretion and endomembrane defects: delayed protein transport and reduced secretion at high temperature; abnormal endomembrane and ER morphology/distribution; mitochondrial defects upon repression; disrupted chitin deposition, septum and bud neck morphology; altered cell shape and mating projections; decreased bipolar budding; abnormal spindle pole body positioning; and impaired cytokinesis. Mutants are hypersensitive to cell wall/membrane stressors and drugs (Calcofluor White, Congo Red, SDS, micafungin, tunicamycin), as well as sirolimus, camptothecin, and benomyl, with decreased starvation resistance; they show increased resistance to acivicin and to Aβ42 at permissive temperature. Anaerobic growth is normal under ergosterol/Tween supplementation. Pheromone production is reduced in sec3-101.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources