Protein Help

SAK1 / YER129W Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
PAK1 5
Protein Product
serine/threonine protein kinase SAK1
Feature Type
ORF , Verified
Paralog
TOS3 8
EC Number
2.7.11.1

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


Parsing response... [0/0]

Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

ExperimentResultReference
half-life47.1 hrChristiano R, et al. (2014)

Protein Abundance

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
1350SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1367SD1 mM hydrogen peroxide1 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1165SD minus nitrogencellular response to nitrogen starvation15 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
2983YEPDcell quiescence7 dflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
1113SDuntreatedquantitative mass spectrometry evidenceS288Cde Godoy LM, et al. (2008)Ho B, et al. (2018)
Showing 1 to 5 of 11 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


sak1-Δ

View all SAK1 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MDRSDKKVNV EEVNVPSNLQ IELEKSGTSS SVSLRSPTKS SATNLAGMAE GARDNASIAS
61 SSVDSLNMLL ERQRVRQLNH PQHQQHISSS LAKTPTTTSS FCSSGSSKNK VKETNRISLT
121 YDPVSKRKVL NTYEIIKELG HGQHGKVKLA RDILSKQLVA IKIVDRHEKK QRKFFTFIKS
181 SKISENDKIK REIAIMKKCH HKHVVQLIEV LDDLKSRKIY LVLEYCSRGE VKWCPPDCME
241 SDAKGPSLLS FQETREILRG VVLGLEYLHY QGIIHRDIKP ANLLISGDGT VKISDFGVSL
301 AASSTNSSDS SESLDELELA KTVGTPAFFA PEMCLGEDAF TRYNLTKENL FRGSCISFMI
361 DIWAVGVTLY CLLFGMLPFF SDFELKLFEK IVNDPLKFPT FKEIQSNKVS KVSCEEEYEM
421 AKDLLLKLLE KNPQKRMTIP AIKKHPFVSW DFDHVPENDE KLLSSVLEQK LRFQCNQTDQ
481 FEPISISKHE LKNAVSGVGK KIKESVLKSI PLKDPSDLSN KNYLHPTETT RGRGDANVIV
541 SEGSVLSNIK ELSANDGCLN TDSDTNININ DDDHYSGDDN DGHLTKRELE RELNKFDDKH
601 EAGNMVNLPI NSSFASLDSF YIDNFAMARM GMSSPEAGDS VSSVPNLPSA PSSTRLGRSP
661 VFSGVTNQPS PIRPVLPQQK SSFCATGRYD KSHNSLLRNS SSHLTSYNSG RPSSRTGRMN
721 SRNQNLPKIP NSLSKISTTK LTELRVPKDS EIPSPAKNPN ADRLRRFPVK KNTKTPAIKD
781 PPRININSSD KSGSKNSPIK SLYQRMKQSK DNSKTFEVRR GNFFSHFNGD DDDSSSQSSV
841 TSSGSESDSE LSSTSSSCTS GTQSRNSSNN NAYSETESLP FEFGVDSEDG SGVLLRDLPN
901 EDQIRPFLDI QPCRRMKVKS SLNLEPPSVS SSSSSSSDED ELILNVGTAG HRRRHNSSKL
961 SELSNSPQKG SNNFMYSNGS VHDSETTITP QNMDDLTLHQ ALSRSQPISK PGPLVLPKRL
1021 DQKKATTETS NLTDIVEFNG NNDHRKDKNF DKVLYSRDLL KDALSSTNAG RRRSIPSNKI
1081 RGRKDASITM STNVGNDEHA RNTSCHGDKG QENGAIKQRT HERSRSLTVA ELNEEKRRSA
1141 LP*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

92 entries for 44 sites

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

SiteModificationModifierReference
S30phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S31phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S31phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S33phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
S33phosphorylated residueMacGilvray ME, et al. (2020) PMID: 32597660
S33phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S33phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S36phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S36phosphorylated residueCDC28Holt LJ, et al. (2009) PMID: 19779198
S36phosphorylated residueZhou X, et al. (2021) PMID: 33481703
Showing 1 to 10 of 92 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A453.94
C151.31
D746.48
E706.13
F393.42
G564.90
H292.54
I574.99
K938.14
L1028.93
M201.75
N796.92
P585.08
Q353.06
R665.78
S16614.54
T595.17
V585.08
W30.26
Y181.58

Physical Details

Length (a.a): 1142
Molecular Weight (Da): 126889.1
Isoelectric Point (pl): 9.01
Formula: C5471H8853N1608O1788S35
Aliphatic Index: 70.96
Instability Index: 52.49

Coding Region Translation Calculations

Codon Bias: -0.04
Codon Adaptation Index: 0.12
Frequence of Optimal Codons: 0.4
Hydropathicity of Protein: -0.72
Aromaticity Score: 0.05

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 44195.0
NO Cys residues appear as half cystines: 43320.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

20 entries for 10 sources


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

External IDSource
orf19.3840CGD
5407DIP
2.7.11.1ExPASy
U13398GenBank/EMBL/DDBJ
U18916GenBank/EMBL/DDBJ
AAC03227.1GenBank/EMBL/DDBJ
AAC49840.1GenBank/EMBL/DDBJ
730268GenBank/EMBL/DDBJ
398364785GenBank/EMBL/DDBJ
603368GenBank/EMBL/DDBJ
Showing 1 to 10 of 20 entries

Resources