YAP3 / YHL009C Overview


Standard Name
YAP3 1
Systematic Name
YHL009C
SGD ID
SGD:S000001001
Feature Type
ORF , Verified
Description
Basic leucine zipper (bZIP) transcription factor 1
Name Description
Yeast AP-1 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
330
Mol. Weight (Da)
37942.5
Isoelectric Point
4.77
Median Abundance (molecules/cell)
1947 +/- 624
Half-life (hr)
2.9

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all YAP3 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Sequence-specific DNA binding transcription factor involved in the regulation of transcription by RNA polymerase II

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null exhibits abnormal growth and vacuolar morphology, decreased resistance hyperosmotic stress, decreased fitness in alkaline pH; null shows increased resistance to acid and DNA-damaging agents; overexpression confers resistance to TOR inhibitor rapamycin; homozygous diploid null has increased cell size, accumulates glycogen, is sensitive to zinc, caffeine, heat, dessication, rapamycin, and glycosylation inhibitor tunicamycin
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


82 total interactions for 76 unique genes

Physical Interactions

  • Affinity Capture-MS: 2
  • Affinity Capture-RNA: 4
  • Biochemical Activity: 7
  • Cross-Linking-MS (XL-MS): 1
  • PCA: 1
  • Proximity Label-MS: 3
  • Reconstituted Complex: 2
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Negative Genetic: 50
  • Positive Genetic: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
YAP3 encodes an AP-1 type transcription factor which contains a basic leucine zipper domain (bZIP), and activates transcription from promoters containing a Yap recognition element (YRE; 5'-TTAC/GTAA-3'). Little is known about the regulatory targets of Yap3p, but it appears to play a specific role in the cellular response to hydroquinone (HQ). Like other Yap family members, Yap3p contains two cysteine-rich domains (CRD), and also contains a nuclear export signal (NES) in its C-terminus. Yap3p is retained in the nucleus only following treatment with HQ. Yap3p may also respond to ER stress, as null mutants are sensitive to tunicamycin, a compound that causes ER stress through induction of the unfolded protein response. Null mutants are also sensitive to arsenic treatment, and some work suggests that Yap3p sites co-occur with those of Arr1p (Yap8p), the major arsenic response transcription factor.
Regulators
2
Targets
5
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
10
Additional
29
Reviews
7

Resources