DAL81 / YIR023W Overview
- Standard Name
- DAL81
- Systematic Name
- YIR023W
- SGD ID
- SGD:S000001462
- Aliases
- UGA35 3 , DURL 3
- Feature Type
- ORF , Verified
- Description
- Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism 1 2
- Name Description
- Degradation of Allantoin
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 970
- Mol. Weight (Da)
- 109179.7
- Isoelectric Point
- 8.11
- Median Abundance (molecules/cell)
- 1533 +/- 944
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DAL81 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- A RNA polymerase II transcription coactivator involved in activation of transcription from RNA polymerase II promoter, positive regulation of urea catabolic process, and RNA polymerase II transcriptional preinitiation complex assembly; localized to nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables transcription coactivator activity (IMP, IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in positive regulation of gamma-aminobutyric acid catabolic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IPI)
- involved in positive regulation of urea catabolic process (IMP)
- involved in RNA polymerase II preinitiation complex assembly (IDA, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation causes decreased expression of multiple genes controlled by nitrogen catabolite repression system; reduction of function results in decreased ability to utilize urea and gamma-aminobutyrate as nitrogen sources; in systematic studies mutants are sensitive to both acidic and alkaline conditions, also sensitive to numerous chemicals and accumulate free amino acids
Classical Genetics
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- metal resistance: increased
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- RNA accumulation: decreased
- small molecule transport: decreased
- utilization of nitrogen source: decreased
- reduction of function
- null
- acid pH resistance: decreased
- alkaline pH resistance: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- endocytosis: decreased
- filamentous growth: decreased
- haploinsufficient
- heat sensitivity: decreased
- invasive growth: absent
- metal resistance: decreased
- mitotic recombination: decreased
- nutrient utilization: decreased
- protein activity: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: decreased
- toxin resistance: increased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
682 total interactions for 583 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 6
- Biochemical Activity: 1
- Co-localization: 1
- PCA: 1
- Proximity Label-MS: 1
- Two-hybrid: 4
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 1
- Negative Genetic: 510
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 1
- Positive Genetic: 120
- Synthetic Growth Defect: 23
- Synthetic Lethality: 4
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- DAL81 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Dal81p is required for transcriptional activation of many genes involved in nitrogen source utilization, always in cooperation with another transcription factor specific for a particular nitrogen source. It has regulates utilization of gamma-aminobutyrate (GABA), together with Uga3p; allantoin, with Dal82p; and amino acids, with Stp1p and Stp2p. This common requirement for Dal81p in the pathways for utilization of several different nitrogen sources allows the cell to integrate the disparate nutritional signals and to induce different pathways according to availability of each source. Although Dal81p has a putative DNA-binding domain, it is unclear whether it binds DNA in vivo, since this domain is not required for transcriptional activation with at least some of its partners (Uga3p and Dal82p). Furthermore, Dal81p has been shown to interact physically with Dal82p, so its mechanism of action may involve binding to its partner transcription factor rather than to DNA.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.