GCN3 / YKR026C Overview
- Standard Name
- GCN3 1
- Systematic Name
- YKR026C
- SGD ID
- SGD:S000001734
- Aliases
- AAS2 17 , eIF2Bα
- Feature Type
- ORF , Verified
- Description
- Alpha subunit of translation initiation factor eIF2B (eIF2Bα); guanine-nucleotide exchange factor for eIF2; mutations in eIF2Bα alter localization of eIF2B; activity regulated by phosphorylated eIF2; positive regulator of GCN4 expression; mutations in human ortholog EIF2B2 cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); human ortholog EIF2B1 can complement yeast null mutant 2 4 5 6 7
- Name Description
- General Control Nonderepressible 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 305
- Mol. Weight (Da)
- 34015.4
- Isoelectric Point
- 4.97
- Median Abundance (molecules/cell)
- 4129 +/- 1186
- Half-life (hr)
- 13.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GCN3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Translation initiation factor that contributes to the exchange of guanyl nucleotides associated with GTPases; localizes to translation initiation factor 2B complex
View computational annotations
Molecular Function
- Manually Curated
- enables enzyme regulator activity (IGI, IMP)
- contributes to guanyl-nucleotide exchange factor activity (IDA)
- enables translation initiation factor activity (IMP, IDA)
Biological Process
- Manually Curated
- involved in positive regulation of cellular response to amino acid starvation (IMP, IGI)
- involved in positive regulation of cellular response to heat (IMP)
- involved in positive regulation of translational initiation (IMP)
- involved in regulation of translational initiation (IGI, IMP, IPI, IDA)
Cellular Component
- Manually Curated
- located in cytosol (HDA)
- part of eukaryotic translation initiation factor 2B complex (IMP, IDA, IPI)
- part of guanyl-nucleotide exchange factor complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows increased sensitivity to numerous drugs, decreased thermotolerance and reduced competitive fitness; homozygous diploid is auxotrophic for inositol; overexpression causes slow growth
Classical Genetics
- null
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- competitive fitness: increased
- endocytosis: decreased
- haploinsufficient
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: increased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast GCN3 is orthologous to human EIF2B1, and has been used to study the fatal brain disorder childhood ataxia with central nervous system hypomyelination (CACH), also known as vanishing white matter leukoencephalopathy (VWM)
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
327 total interactions for 192 unique genes
Physical Interactions
- Affinity Capture-MS: 92
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 13
- Co-fractionation: 3
- Co-localization: 2
- Co-purification: 2
- PCA: 2
- Reconstituted Complex: 2
- Two-hybrid: 14
Genetic Interactions
- Dosage Rescue: 6
- Negative Genetic: 123
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 10
- Positive Genetic: 32
- Synthetic Growth Defect: 8
- Synthetic Lethality: 6
- Synthetic Rescue: 7
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-11-28
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.