Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
49 entries for 20 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| alkaline pH resistance: decreased | systematic mutation set | null Allele: cak1-Δ | S288C | 60 mM calcium dichloride | Media: alkaline conditions, pH 7.5 Media: YPD Details: growth defects at pH 7.5 in 60 mM calcium chloride - vma phenotype | Sambade M, et al. (2005) PMID:15937126 |
| bud morphology: abnormal | classical genetics | reduction of function Allele: cak1-P212S | Other | Details: multiple, elongated and branched buds | Schmidt M, et al. (2008) PMID:18373684 | |
| cell cycle progression in G2 phase: delayed | classical genetics | conditional Allele: cak1-22 EKG313-315 to AA; nucleotides G937–T945 (GAGAAAGAT) mutated to GCCGCG; loop L14 mutated | W303 | Temperature: elevated temperature, 37 °C Details: majority of budded cells, were enlarged, had a short spindle spanning an undivided nucleus at the mother/bud neck, and an enrichment of cells with a 2N DNA content | Kaldis P, et al. (1996) PMID:8752210 | |
| cellular morphology: abnormal | classical genetics | conditional Allele: cak1-17 | SK1 | Temperature: permissive temperature, 26 °C Details: display highly elongated cells that remain attached to each other forming clumps at both permissive and elevated temperatures when present at low copy number, in contrast to high copy number cak1-17 | Wagner M, et al. (1997) PMID:9135146 | |
| cellular morphology: abnormal | classical genetics | overexpression Allele: cak1-17 | SK1 | Temperature: elevated temperature, 34 °C Details: arrest as large unbudded cells at elevated temperature when cak1-17 is present at high copy number, in contrast to low copy cak1-17 | Wagner M, et al. (1997) PMID:9135146 | |
| cellular morphology: abnormal | classical genetics | conditional Allele: cak1-22 EKG313-315 to AA; nucleotides G937–T945 (GAGAAAGAT) mutated to GCCGCG; loop L14 mutated | W303 | Temperature: elevated temperature, 37 °C Details: 92% of budded cells were elongated, had elongated buds or both after 7 hours | Kaldis P, et al. (1996) PMID:8752210 | |
| cellular morphology: abnormal | classical genetics | conditional Allele: cak1-95 G143E (G428A); α-Helix 3 mutated | W303 | Temperature: elevated temperature, 37 °C Details: majority arrest as unbudded cells with a small fraction of large budded cells containing elongated buds and a single DNA mass | Espinoza FH, et al. (1998) PMID:9774652 | |
| cellular morphology: normal | classical genetics | overexpression Allele: cak1-17 | SK1 | Temperature: permissive temperature, 26 °C Details: displays wt morphology at the permissive temperature when cak1-17 is present at high copy number, in contrast to low copy number cak1-17 | Wagner M, et al. (1997) PMID:9135146 | |
| chromosome/plasmid maintenance: decreased | heterozygous diploid, systematic mutation set | null Allele: cak1-Δ | S288C | Assay: diploid bimater (BiM) assay Details: haploinsufficiency results in chromosome instability (CIN); scored >= 2 standard deviations above the mean | Choy JS, et al. (2013) PMID:23825022 | |
| cytokinesis: abnormal | classical genetics | reduction of function Allele: cak1-P212S | Other | Schmidt M, et al. (2008) PMID:18373684 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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