KNH1 / YDL049C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

KNH1/YDL049C is a non-essential gene in reference strain S288C, and null mutants are viable but exhibit various phenotypic abnormalities. Null mutants show decreased chromosome/plasmid maintenance with haploinsufficiency resulting in chromosome instability (CIN), scored significantly higher than the mean in a diploid bimater assay. They also demonstrate decreased mitotic recombination with less than 33% recombination frequency compared to 56% for wild type, as identified using a high-throughput replica-pinning technique. These mutants have decreased cell size, falling into the smallest 5% of haploid deletion strains, and show reduced acid pH resistance to propionic acid (20mM). Additional abnormalities include abnormal vacuolar morphology under sodium chloride (0.4M), increased sensitivity to chemicals such as triclosan (15μg/ml) and 2,4-dichlorophenol (0.3mM), significantly altered free amino acid profiles, and increased resistance to the chemical edelfosine (60μM). Overexpression of KNH1 results in decreased vegetative growth, and null mutants exhibit decreased rates of vegetative growth, abnormal mitochondrial genome maintenance with increased mitochondrial DNA copy number, increased oxidative stress resistance to paraquat (1mM), increased competitive fitness under conditions mimicking Phase I of a batch fermentation, decreased starvation resistance, and a decreased chronological lifespan.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

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