ARF1 / YDL192W Overview
- Standard Name
- Systematic Name
- SGD ID
- Feature Type
ADP-ribosylation factor; GTPase of the Ras superfamily that regulates coated vesicle formation during intracellular trafficking within the Golgi; regulates mitochondrial fission, fusion and function, controlling the flow of fatty acids and metabolites from lipid droplets to peroxisomes and mitochondria; localizes to the Golgi apparatus and the active form to ER and lipid droplets; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
- Name Description
- ADP-Ribosylation Factor
- Comparative Info
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
- ARF1 has a paralog, ARF2, that arose from the whole genome duplication
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- Mol. Weight (Da)
- Isoelectric Point
- Median Abundance (molecules/cell)
- 33643 +/- 14001
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
Gene Ontology Details
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
- GTPase that acts as ADP ribosylation factor (ARF); involved regulation of vesicle formation in ER to Golgi and Golgi to plasma membrane vesicular transport, and in macroautophagy; localizes in Golgi
View computational annotations
- Manually Curated
- Manually Curated
- Manually Curated
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
- Non-essential gene; null mutant is temperature sensitive for growth, is a myo-inositol auxotroph, and displays decreased competitive fitness and growth rate; null mutant has protein processing/trafficking defects, and displays decreased replicative and chronological lifespans, survival rate in stationary phase, mitophagy, desiccation resistance and shortened telomeres; overexpression results in a G2 or M phase delay
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
- The arf1 null mutant is viable; the null mutant of paralog arf2 is viable; the arf1 arf2 double mutant is inviable.
740 total interactions for 389 unique genes
- Affinity Capture-MS: 44
- Affinity Capture-RNA: 11
- Affinity Capture-Western: 32
- Biochemical Activity: 3
- Co-crystal Structure: 4
- Co-localization: 3
- Co-purification: 6
- PCA: 36
- Proximity Label-MS: 1
- Reconstituted Complex: 19
- Two-hybrid: 8
- Dosage Growth Defect: 3
- Dosage Lethality: 2
- Dosage Rescue: 21
- Negative Genetic: 325
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 83
- Positive Genetic: 71
- Synthetic Growth Defect: 23
- Synthetic Lethality: 31
- Synthetic Rescue: 7
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
- ARF1 encodes a small GTPase of the Ras superfamily that acts as an ADP ribosylation factor with a critical role in control of membrane traffic. ARF1 has a paralog, ARF2, that appears to be functionally interchangeable with ARF1. The activity of Arf1p or Arf2p (ARF) is essential to initiate the formation of COPI vesicles at the Golgi membrane that carry out retrograde transport between the Golgi and the endoplasmic reticulum, and within the Golgi compartments. ARF exists in an inactive GDP-bound form in the cytosol and an active GTP-bound form associated with the membranes. The exchange of GDP for GTP on ARF is catalyzed by guanine nucleotide exchange factors (GEFs) Sec7p, Gea1p, Gea2p, and Syt1p. Activation of ARF triggers a conformational change that exposes its N-terminal domain to be inserted into membranes. Membrane-bound GTP-ARF then stimulates the recruitment of COPI coat proteins (Cop1p, Ret2p, Ret3p, Sec21p, Sec26p, Sec27p) and formation of the COPI vesicle. The subsequent steps of vesicle budding and delivery to its target membrane require inactivation of ARF, i.e. hydrolysis of ARF-bound GTP. Since ARF has very little intrinsic GTPase activity, this step depends on GTPase-activating proteins (GAPs) Gcs1p and Glo3p. The precise mechanisms that control GEFs and GAPs functions and thereby impact ARF activity remain to be elucidated. The entire control system is highly conserved throughout evolution and human ARF genes (ARF1, ARF4, ARF5, or ARF6) can complement inactivation of ARF1 and ARF2 genes in yeast.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.