Protein Help

ASF2 / YDL197C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Feature Type
ORF , Verified

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


Parsing response... [0/0]

Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

ExperimentResultReference
half-life4.3 hrChristiano R, et al. (2014)

Protein Abundance

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
4405YEPDcell quiescence7 dflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
1910YEPDuntreatedqualitative western immunoblotting evidenceS288CGhaemmaghami S, et al. (2003)Ho B, et al. (2018)
569YEPDuntreatedquantitative mass spectrometry evidenceOtherLu P, et al. (2007)Ho B, et al. (2018)
Showing 1 to 3 of 3 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


asf2-Δ | asf2::HIS3

View all ASF2 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MPKNRGVLDA ITRSVIDGSD QESSSSFNSD KEYAAVTKGL SSSRVQKKSS LRQMKSKVKE
61 LQSLVNHYRE NEAALVSSAK LLSGEIIGYE IKMASLHGKM KSILDENNAL KETHKSSAEK
121 RIELVRLPSS KEERNYDEYT LLVNLKKEIC AKLQDYKNVQ NTVNTKLDEI HTFHEKYYEG
181 LELSLDSKVF DAESSKELAK VRRELNNVRK NSEIKVNNLK MQLLQATKSL EHLKKQAKAK
241 DDYLKCIPEL VDKANLTMLS YKKSIANQRE TIEALQAELS QQSETKGQIE TETQNQVQIP
301 TNVTLVDPFE ENNPEDLFAI QEQELQDLRL HKKMADERSR TTHLHLERKN NTIKLLQSYV
361 QSLIQRLPPA QRKHHLGIFQ KLGSEKSCPL APAVASTYAP LLLLSQHSNH QEIDNTPQRL
421 LLAAPDGQSY SEKSTTLNLD YSSRKSYLSR LQPPHIANLK SLTLKTLPRV PTDSPQLPSK
481 DKSQETAKKD DRPKLVANEP VTLDTSTPPV AQSLADSKHC SGLHK*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

23 entries for 21 sites

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

SiteModificationModifierReference
S29phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
K38sumoylated lysineBhagwat NR, et al. (2021) PMID: 33502312
S83phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
Y89phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
Y89phosphorylated residueHolt LJ, et al. (2009) PMID: 19779198
S95phosphorylated residueHolt LJ, et al. (2009) PMID: 19779198
S95phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S129phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
K131sumoylated lysineBhagwat NR, et al. (2021) PMID: 33502312
K146sumoylated lysineBhagwat NR, et al. (2021) PMID: 33502312
Showing 1 to 10 of 23 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A336.29
C40.76
D264.95
E438.19
F61.14
G122.29
H163.05
I214.00
K5610.67
L6812.95
M71.33
N295.52
P234.38
Q336.29
R234.38
S5610.67
T295.52
V254.76
W00.00
Y152.86

Physical Details

Length (a.a): 525
Molecular Weight (Da): 59358.2
Isoelectric Point (pl): 9.48
Formula: C2587H4262N744O826S11
Aliphatic Index: 82.23
Instability Index: 54.7

Coding Region Translation Calculations

Codon Bias: 0.04
Codon Adaptation Index: 0.12
Frequence of Optimal Codons: 0.45
Hydropathicity of Protein: -0.76
Aromaticity Score: 0.04

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 22600.0
NO Cys residues appear as half cystines: 22350.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

21 entries for 5 sources


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through its pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

External IDSource
1004298GenBank/EMBL/DDBJ
Z74245GenBank/EMBL/DDBJ
L07649GenBank/EMBL/DDBJ
AAC37513.1GenBank/EMBL/DDBJ
CAA98774.1GenBank/EMBL/DDBJ
CAA58250.1GenBank/EMBL/DDBJ
CAA67472.1GenBank/EMBL/DDBJ
6320004GenBank/EMBL/DDBJ
X99000GenBank/EMBL/DDBJ
X83276GenBank/EMBL/DDBJ
Showing 1 to 10 of 21 entries

Resources