REG1 / YDR028C Overview
- Standard Name
- REG1 1
- Systematic Name
- YDR028C
- SGD ID
- SGD:S000002435
- Aliases
- HEX2 13 14 , PZF240 15 , SPP43 16 , SRN1 17 18 19
- Feature Type
- ORF , Verified
- Description
- Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication 2 3 4 5 6
- Name Description
- REsistance to Glucose repression 1
- Paralog
- REG2 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- REG1 has a paralog, REG2, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1014
- Mol. Weight (Da)
- 112556.0
- Isoelectric Point
- 4.68
- Median Abundance (molecules/cell)
- 4140 +/- 1361
- Half-life (hr)
- 7.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all REG1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein phosphatase regulator; subunit of REG1-GLC7 phosphatase complex that plays a role in glucose repression; involved in response to unfolded protein, response to glucose starvation and negative regulation of DNA-binding transcription factors; localized to the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein phosphatase regulator activity (TAS)
Biological Process
- Manually Curated
- involved in cellular response to glucose starvation (IMP)
- involved in protein catabolic process in the vacuole (IDA, IMP)
- involved in response to unfolded protein (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- part of protein phosphatase type 1 complex (TAS)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows decreased rate of fermentative growth, sensitivity to starvation and elevated glycogen accumulation; null mutation decreases utilization of galactose, maltose, sucrose as carbon sources, also increases resistance to a toxic glucose analog 2-deoxy-D-glucose and causes sensitivity to L-canavanine; null mutants have large cell size
Classical Genetics
- unspecified
- null
- cell size: increased
- chemical compound accumulation: increased
- desiccation resistance: increased
- fermentative growth: decreased rate
- invasive growth: increased
- lipid particle morphology: abnormal
- protein activity: increased
- protein transport: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- pseudohyphal growth: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- RNA accumulation: decreased
- RNA accumulation: increased
- silencing: decreased
- small molecule transport: decreased
- starvation resistance: decreased
- utilization of carbon source: decreased
- viable
- conditional
- reduction of function
- null
- alkaline pH resistance: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- haploproficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- lipid particle morphology: abnormal
- nutrient utilization: decreased
- oxidative stress resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: increased rate
- starvation resistance: decreased
- stress resistance: decreased
- toxin resistance: decreased
- toxin resistance: increased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The reg1 null mutant is viable; the null mutant of paralog reg2 is viable; the reg1 reg2 double mutant displays a synthetic growth defect.
258 total interactions for 147 unique genes
Physical Interactions
- Affinity Capture-MS: 70
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 22
- Biochemical Activity: 2
- Co-localization: 1
- PCA: 2
- Proximity Label-MS: 1
- Reconstituted Complex: 3
- Two-hybrid: 14
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 2
- Dosage Rescue: 9
- Negative Genetic: 12
- Phenotypic Enhancement: 4
- Phenotypic Suppression: 21
- Positive Genetic: 35
- Synthetic Growth Defect: 8
- Synthetic Lethality: 10
- Synthetic Rescue: 31
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2005-10-03
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.