RRP8 / YDR083W Overview
- Standard Name
- RRP8 1
- Systematic Name
- YDR083W
- SGD ID
- SGD:S000002490
- Feature Type
- ORF , Verified
- Description
- Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with gar1 mutation; deletion disrupts telomere maintenance by influencing expression of neighboring gene STN1; mammalian homolog nucleomethylin has been linked to nutrient availability signaling pathways and obesity 1 2 3 4 5
- Name Description
- Ribosomal RNA Processing 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rrp8p is 392 amino acids long, shorter-lived, low in abundance; succinylated on K196, acetylated on K68 and K381, phosphorylated on 5 residues
- Length (a.a.)
- 392
- Mol. Weight (Da)
- 46004.8
- Isoelectric Point
- 10.1
- Median Abundance (molecules/cell)
- 3935 +/- 1159
- Half-life (hr)
- 7.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RRP8 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- rRNA methyltransferase involved in rRNA processing and biosynthesis of the large ribosomal subunit
View computational annotations
Molecular Function
- Manually Curated
- enables rRNA (adenine) methyltransferase activity (IMP)
Biological Process
- Manually Curated
- involved in ribosomal large subunit biogenesis (IGI)
- involved in rRNA methylation (IMP)
- involved in rRNA processing (IMP, IGI)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is among smallest 5% of haploid deletion strains, shows small defect in vacuolar fragmentation, increased resistance to the toxicity of Aβ42, decreased chronological lifespan, increased oxidative stress resistance, decreased desiccation resistance, slow cell cycle progression at G1, slow growth, long telomeres, increased resistance to bleomycin, mycophenolic acid, MMS, hydroxyurea, tunicamycin, fenpropimorph, caffeine, decreased resistance to paromomycin, actinomycin D, and Calcofluor White,
Classical Genetics
- null
- reduction of function
- null
- cell cycle progression in G1 phase: increased duration
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- innate thermotolerance: increased
- killer toxin resistance: decreased
- metal resistance: increased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: increased rate
- stress resistance: increased
- telomere length: increased
- toxin resistance: decreased
- toxin resistance: increased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- repressible
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rrp8p interacts physically with proteins involved in rRNA processing; RRP8 interacts genetically with genes involved in rRNA processing
512 total interactions for 389 unique genes
Physical Interactions
- Affinity Capture-MS: 101
- Affinity Capture-RNA: 5
- Co-localization: 1
- Proximity Label-MS: 1
Genetic Interactions
- Dosage Lethality: 2
- Negative Genetic: 291
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 1
- Positive Genetic: 94
- Synthetic Growth Defect: 9
- Synthetic Lethality: 3
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.