MUS81 / YDR386W Overview
- Standard Name
- MUS81 1
- Systematic Name
- YDR386W
- SGD ID
- SGD:S000002794
- Aliases
- SLX3
- Feature Type
- ORF , Verified
- Description
- Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA and DNA-protein cross-links; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates function of Mms4p-Mus81p 1 2 3 4 5 6 7 8
- Name Description
- MMS and UV Sensitive 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 632
- Mol. Weight (Da)
- 72318.7
- Isoelectric Point
- 8.68
- Median Abundance (molecules/cell)
- 576 +/- 317
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MUS81 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Enzyme inhibitor subunit of Holliday junction resolvase complex that contributes to crossover junction endodeoxyribonuclease activity; involved in cellular response to DNA damage stimulus, resolution of meiotic recombination intermediates, DNA topological changes and double-strand break repair via break-induced replication
View computational annotations
Molecular Function
- Manually Curated
- contributes to crossover junction DNA endonuclease activity (IDA)
- contributes to DNA endonuclease activity (IDA)
- enables enzyme inhibitor activity (IDA)
Biological Process
- Manually Curated
- involved in DNA damage response (IMP)
- involved in DNA topological change (IGI)
- involved in double-strand break repair via break-induced replication (IGI, IMP)
- involved in reciprocal meiotic recombination (IGI)
- involved in resolution of meiotic recombination intermediates (IGI)
Cellular Component
- Manually Curated
- part of Holliday junction resolvase complex (IDA)
- located in nucleus (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- apoptosis: decreased
- cell cycle progression in G1 phase: decreased duration
- cell cycle progression in S phase: decreased duration
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased rate
- gamma ray resistance: decreased
- mutation frequency: increased rate
- resistance to chemicals: decreased
- null
- biofilm formation: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- gamma ray resistance: decreased
- haploproficient
- heat sensitivity: increased
- invasive growth: absent
- mitotic recombination: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: increased
- respiratory growth: increased rate
- sporulation efficiency: decreased
- sporulation: normal
- UV resistance: decreased
- vacuolar morphology: abnormal
- viable
- X ray resistance: decreased
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
567 total interactions for 254 unique genes
Physical Interactions
- Affinity Capture-MS: 71
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 9
- Biochemical Activity: 2
- Co-crystal Structure: 1
- Co-localization: 6
- Co-purification: 2
- Reconstituted Complex: 14
- Two-hybrid: 9
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 5
- Negative Genetic: 218
- Phenotypic Enhancement: 29
- Phenotypic Suppression: 14
- Positive Genetic: 24
- Synthetic Growth Defect: 100
- Synthetic Lethality: 46
- Synthetic Rescue: 11
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 7
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.