NPL3 / YDR432W Overview
- Standard Name
- NPL3 1
- Systematic Name
- YDR432W
- SGD ID
- SGD:S000002840
- Aliases
- NAB1 13 , MTR13 14 , MTS1 15 , NOP3 16
- Feature Type
- ORF , Verified
- Description
- RNA-binding guard protein; promotes transcription elongation and regulates termination; binds eIF4G to repress translation initiation; role in nuclear export of poly(A) mRNA and in pre-mRNA splicing; links chromatin modification to mRNA processing; prevents R-loop-mediated genome instability and replication stress; binds TERRA, stabilizing R-loops at short telomeres; phosphorylated by Sky1p to promote shuttling and mRNA dissociation; protein abundance increases during DNA replication stress 2 3 4 5 6 7 8 9 10 11 12
- Name Description
- Nuclear Protein Localization 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 414
- Mol. Weight (Da)
- 45393.9
- Isoelectric Point
- 5.25
- Median Abundance (molecules/cell)
- 34936 +/- 26291
- Half-life (hr)
- 9.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all NPL3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- RNA-binding protein involved in pre-mRNA splicing and mRNA export from the nucleus; regulates RNA pol II transcription elongation and termination; implicated in translation termination and repression of translational initiation; shuttles between the cytoplasm and nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables double-stranded telomeric DNA binding (IDA)
- enables eukaryotic initiation factor 4G binding (IDA)
- enables mRNA binding (IDA, HDA)
- enables poly(A) binding (IDA)
- enables RNA polymerase II complex binding (IPI)
- enables telomeric repeat-containing RNA binding (IDA)
Biological Process
- Manually Curated
- involved in mRNA splicing, via spliceosome (IMP, IGI)
- involved in negative regulation of termination of RNA polymerase II transcription, poly(A)-coupled (IDA, IMP)
- involved in negative regulation of translation (IDA)
- involved in positive regulation of transcription elongation by RNA polymerase II (IDA, IMP)
- involved in translational termination (IGI, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene in S288C, reported essential in Sigma1278b; null mutant is sensitive to elevated temperatures, metals and multiple drugs, including rapamycin, paromomycin, tacrolimus; in systematic studies homozygous null mutant diploid shows slow growth, decreased fermentative growth, inositol auxotrophy, decreased innate thermotolerance, abnormal budding pattern and elevated sensitivity to multiple drugs, including doxorubicin, echinocandin, fenpropimorph, hygromycin B
Classical Genetics
- conditional
- null
- overexpression
- unspecified
- null
- anaerobic growth: decreased
- auxotrophy
- biofilm formation: decreased
- budding pattern: abnormal
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- fermentative growth: decreased rate
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: decreased
- inviable
- killer toxin resistance: decreased
- metal resistance: decreased
- metal resistance: increased
- mitotic recombination: decreased
- nutrient utilization: decreased
- resistance to chemicals: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: increased
- transposable element transposition: decreased
- transposable element transposition: increased
- utilization of phosphorus source: decreased
- UV resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased
- vegetative growth: decreased rate
- viable
- overexpression
- repressible
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1595 total interactions for 1214 unique genes
Physical Interactions
- Affinity Capture-Luminescence: 2
- Affinity Capture-MS: 236
- Affinity Capture-RNA: 14
- Affinity Capture-Western: 61
- Biochemical Activity: 20
- Co-localization: 5
- Co-purification: 1
- Far Western: 1
- PCA: 1
- Protein-peptide: 6
- Protein-RNA: 3
- Proximity Label-MS: 1
- Reconstituted Complex: 21
- Two-hybrid: 20
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 9
- Negative Genetic: 72
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 17
- Positive Genetic: 18
- Synthetic Growth Defect: 384
- Synthetic Lethality: 42
- Synthetic Rescue: 656
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.