YHP1 / YDR451C Overview
- Standard Name
- YHP1 1
- Systematic Name
- YDR451C
- SGD ID
- SGD:S000002859
- Feature Type
- ORF , Verified
- Description
- Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication 2 3 4
- Name Description
- Yeast Homeo-Protein 1
- Paralog
- YOX1 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- YHP1 has a paralog, YOX1, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 353
- Mol. Weight (Da)
- 39626.0
- Isoelectric Point
- 10.67
- Median Abundance (molecules/cell)
- 857 +/- 386
- Half-life (hr)
- 21.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all YHP1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription factor involved in the negative regulation of transcription by RNA polymerase II during the G1/S transition of the mitotic cell cycle
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IGI)
- involved in negative regulation of transcription by RNA polymerase II (IGI, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; overexpression impairs growth, budding, and cytoskeletal morphology; haploid null mutants exhibit abnormal vacuolar morphology, impaired fitness and anaerobic growth, increased resistance to DNA-damaging agent methyl methanesulfonate (MMS), and decreased resistance to caffeine; homozygous diploid nulls are sensitive to dessication, zinc limitation, sterol biosynthesis inhibitor fenpropimorph, and antibiotic nigericin; heterozygous diploid null shows increased fitness
Classical Genetics
- null
- anaerobic growth: decreased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- haploproficient
- resistance to chemicals: decreased
- resistance to chemicals: increased
- stress resistance: decreased
- utilization of carbon source: decreased rate
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The yhp1 null mutant is viable; the null mutant of paralog yox1 is viable; the yhp1 yox1 double mutant has not been annotated for phenotype.
94 total interactions for 83 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 1
- Biochemical Activity: 5
- Co-purification: 1
- Protein-RNA: 1
- Reconstituted Complex: 2
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 5
- Dosage Lethality: 1
- Negative Genetic: 50
- Positive Genetic: 15
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- YHP1 encodes a helix-turn-helix transcriptional repressor of the homeodomain family. Yhp1p regulates gene expression that occurs late in the cell cycle, and restricts transcription of genes containing early cell cycle boxes (ECBs) to M-G1 phase. Yhp1p is an ancillary transcription factor that binds alongside Mcm1p on promoters. Examples include important cell cycle genes such as SWI4 and CLN3, themselves rate-limiting for cell cycle progression, and essential for the control of subsequent waves of gene expression at G1-S. Removal of Yhp1p from promoters allows gene expression at M-G1. Transcription of YHP1 is activated by the Hcm1p forkhead-like transcription factor. Yhp1p is expressed later in the cell cycle and helps to maintain ECB repression until late M-phase.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.