Protein Help

HEH2 / YDR458C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Feature Type
ORF , Verified
Paralog
SRC1 3

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life8.2 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
1539SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1314SD2 mM 1,4-dithiothreitol2 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1443SD1 mM hydrogen peroxide1 hrconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
1128SD minus nitrogencellular response to nitrogen starvation15 hr0.73confocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
2297SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
Showing 1 to 5 of 22 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


heh2-Δ

View all HEH2 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MDHRNLDPKT LKVSQLRRVL VENDVAFPAN ARKPVLVKLF EEKVRQRLQS SPEASKVRTS
61 IQKVVKSGAK NADRKKTLKS KKLESSSSES KTVKDENVET NKRKREQIST DNEAKMQIQE
121 EKSPKKKRKK RSSKANKPPE SPPQSKSDGK ATSADLTSEL ETVEELHKKD SSDDKPRVKE
181 LPKPELPNLK VSNEFLAQLN KELASAATEN YDHSIKSTDL SSIRIETEEP VGPSTGAETR
241 NESEVMENIN LEVQPEVKEA KEELTKISET FDNQDEEDTS RLSSKKNIRS PKGRTRHFIA
301 NKTKRGIDIM KPFIAHLFIW LWNGAIFLSI ICPILFGLWY REQRIQVGYC GHEKPLKSLA
361 ISAFPQTERV DSVLQAYRPN CLECPEHGIC SSFMNVECEP GYEPKSSILE TYGIIPFPKY
421 CAKDESKEKE VDELVWKVNE YLKKKNAQHE CGEGENLFES GETETKLYDI FSHSRPSWES
481 QREFNDHWKN VLEILKKKDD IIWLPLDFET NGKREKSKSN NTNYIYRSTS KKWVTLQCHL
541 EGDIQEYITK YGGSLFITLG VLFLIKKIQS TLDNYVQGEQ IIEKLVKEAI DKLKDVKKNK
601 GEEPFLTTVQ LRATLLSDIP NIKEQNNLWA QTKEKIMKEQ SENIELYLLE ENGEIMTCWE
661 WKE*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

55 entries for 24 sites

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SiteModificationModifierReference
S51phosphorylated residueLeutert M, et al. (2023) PMID: 37845410
S51phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S51phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
S51phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S51phosphorylated residueMacGilvray ME, et al. (2020) PMID: 32597660
S55phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S55phosphorylated residueMacGilvray ME, et al. (2020) PMID: 32597660
S109phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
T110phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
T110phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
Showing 1 to 10 of 55 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A274.07
C101.51
D304.52
E7911.92
F192.87
G243.62
H111.66
I426.33
K8212.37
L578.60
M71.06
N375.58
P314.68
Q263.92
R294.37
S588.75
T355.28
V334.98
W111.66
Y152.26

Physical Details

Length (a.a): 663
Molecular Weight (Da): 76379.6
Isoelectric Point (pl): 7.91
Formula: C3374H5420N928O1053S17
Aliphatic Index: 72.13
Instability Index: 50.8

Coding Region Translation Calculations

Codon Bias: -0.02
Codon Adaptation Index: 0.13
Frequence of Optimal Codons: 0.43
Hydropathicity of Protein: -0.85
Aromaticity Score: 0.07

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 83475.0
NO Cys residues appear as half cystines: 82850.0

Atomic Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

18 entries for 8 sources


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External IDSource
7259DIP
U33050GenBank/EMBL/DDBJ
AAB64934.1GenBank/EMBL/DDBJ
6320666GenBank/EMBL/DDBJ
2497055GenBank/EMBL/DDBJ
927762GenBank/EMBL/DDBJ
922664593GenBank/EMBL/DDBJ
922664592GenBank/EMBL/DDBJ
1876LoQAtE
NM_001180766.1NCBI
Showing 1 to 10 of 18 entries

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