PIB2 / YGL023C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

PIB2/YGL023C is a non-essential gene in reference strain S288C, deletion mutants are viable but show reduced vegetative and competitive growth, including poorer anaerobic growth and sensitivity to hydrostatic pressure and oxidative stress (paraquat). pib2Δ alters metabolism and protein homeostasis, with increased alanine/valine (and glutamine under high pressure), increased glycogen, decreased serine and H2S (with cysteine), and impaired protein modification/redistribution under nitrogen starvation and cycloheximide. The mutant is broadly drug-sensitive, notably to caffeine, alcohols (ethanol, isobutanol, propanol), myriocin, aflatoxin B1, doxorubicin, diethyl phthalate, and rapamycin/sirolimus (with FYVE and conserved residue mutants also sirolimus‑sensitive), and shows reduced killer toxin resistance; resistance to wortmannin is unaffected. Conversely, pib2Δ increases resistance to Aβ42 (with reduced toxin‑induced cell death), tacrolimus, tunicamycin, DTT, poacic acid, polygodial, and several azole fungicides. Overexpression further decreases growth and tolerance to caffeine and sirolimus and promotes invasive growth.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources