DST1 / YGL043W Overview
- Standard Name
- DST1 1
- Systematic Name
- YGL043W
- SGD ID
- SGD:S000003011
- Aliases
- P37 8 , TFIIS 9 , S-II 10 , SII 9 , PPR2 11
- Feature Type
- ORF , Verified
- Description
- General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress 2 3 4 5 6 7
- Name Description
- DNA Strand Transfer 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 309
- Mol. Weight (Da)
- 34853.0
- Isoelectric Point
- 9.26
- Median Abundance (molecules/cell)
- 7193 +/- 2692
- Half-life (hr)
- 9.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all DST1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Core RNA polymerase II-recruiting, regulatory region DNA-binding transcription factor involved in transcription initiation, elongation, and antitermination; involved in the maintenance of transcriptional fidelity and regulation of mRNA 3-end processing; also involved in transcription from RNA pol I and III promoters; localizes to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables RNA polymerase II complex binding (IPI)
- enables RNA polymerase II complex recruiting activity (IMP)
- enables RNA polymerase II transcription regulatory region sequence-specific DNA binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II (IGI, IMP)
- involved in positive regulation of RNA polymerase II transcription preinitiation complex assembly (IMP, IDA)
- involved in positive regulation of transcription elongation by RNA polymerase II (IDA)
- involved in regulation of mRNA 3'-end processing (IGI, IMP)
- involved in transcription antitermination (IDA)
- involved in transcription by RNA polymerase III (IDA)
- involved in transcription elongation by RNA polymerase I (IDA)
- involved in transcription elongation by RNA polymerase II (IDA, IMP)
- involved in transcription initiation at RNA polymerase II promoter (IGI, IMP, IDA)
- involved in tRNA transcription by RNA polymerase III (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has lower abundance of Polymerase II and exhibits increased sensitivity to toxic nucleobase analogs, azauridine and azauracil; in systematic studies mutants show reduced competitive fitness, haploinsufficiency, and elevated sensitivity to multiple drugs, including fluconazole, bleomycin, hygromycin B; as well as caffeine, cycloheximide, fenpropimorph; mutants also show elevated resistance to tunicamycin and MMS
Classical Genetics
- null
- acid pH resistance: decreased
- chemical compound accumulation: abnormal
- competitive fitness: decreased
- haploinsufficient
- heat sensitivity: increased
- innate thermotolerance: decreased
- liquid culture appearance: abnormal
- mitotic recombination: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- stress resistance: increased
- toxin resistance: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
933 total interactions for 605 unique genes
Physical Interactions
- Affinity Capture-MS: 36
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 10
- Biochemical Activity: 1
- Co-purification: 1
- PCA: 3
- Reconstituted Complex: 13
- Two-hybrid: 4
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 5
- Negative Genetic: 534
- Phenotypic Enhancement: 13
- Phenotypic Suppression: 14
- Positive Genetic: 133
- Synthetic Growth Defect: 84
- Synthetic Lethality: 57
- Synthetic Rescue: 20
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 8
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.