VPS45 / YGL095C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

VPS45/YGL095C is a non-essential gene; null mutants are viable and exhibit a range of phenotypes that significantly affect cellular functions and overall organismal fitness. Mutants lacking this gene exhibit decreased resistance to both acidic and alkaline pH levels, indicating a reduced ability to survive in varying environmental conditions. Additionally, these mutants display an abnormal budding pattern and irregular cell shape, which may disrupt normal cellular processes and division. Competitive fitness is notably diminished in VPS45 mutants, leading to challenges in resource acquisition and survival in competitive environments. Their resistance to desiccation, freeze-thaw cycles, and hydrostatic pressure is also decreased, highlighting vulnerabilities to extreme environmental stresses. Furthermore, these mutants show impaired endocytosis and abnormal morphology of the endomembrane system, which could affect nutrient uptake and intracellular transport. Resistance to hyperosmotic and ionic stress is reduced, as is their ability to withstand oxidative stress, further compromising their resilience. Mitochondrial morphology is abnormal in these mutants, potentially impacting energy production and metabolism. The rate of respiratory growth is decreased, and their ability to resist starvation is also diminished, indicating a reliance on specific metabolic pathways that may be disrupted. Additionally, vacuolar morphology is abnormal, which may affect cellular storage and waste management. Despite these challenges, VPS45 mutants remain viable, although their overall fitness and adaptability are significantly impaired.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources