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SNF4 / YGL115W Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.

Aliases
CAT3 10 , SCI1 53
Protein Product
AMP-activated serine/threonine-protein kinase regulatory subunit SNF4
Feature Type
ORF , Verified
Summary
Protein abundance increases in response to DNA replication stress

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.

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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.

Protein Half Life

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ExperimentResultReference
half-life11.6 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
1348SDuntreatedconfocal microscopy evidenceS288CBreker M, et al. (2013)Ho B, et al. (2018)
3910SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
3373SD0.2 M hydroxyurea160 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
2592SD200 ng/ml sirolimus700 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
6015YEPDuntreatedflow cytometry evidenceS288CDavidson GS, et al. (2011)Ho B, et al. (2018)
Showing 1 to 5 of 28 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.

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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

SNF4-N177S | snf4-319 | snf4-C136A | snf4-C136Y | snf4-E269* | snf4-G145D | snf4-G145E | ... Show all

View all SNF4 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence. 

  1 MKPTQDSQEK VSIEQQLAVE SIRKFLNSKT SYDVLPVSYR LIVLDTSLLV KKSLNVLLQN 
 61 SIVSAPLWDS KTSRFAGLLT TTDFINVIQY YFSNPDKFEL VDKLQLDGLK DIERALGVDQ 
121 LDTASIHPSR PLFEACLKML ESRSGRIPLI DQDEETHREI VVSVLTQYRI LKFVALNCRE 
181 THFLKIPIGD LNIITQDNMK SCQMTTPVID VIQMLTQGRV SSVPIIDENG YLINVYEAYD 
241 VLGLIKGGIY NDLSLSVGEA LMRRSDDFEG VYTCTKNDKL STIMDNIRKA RVHRFFVVDD 
301 VGRLVGVLTL SDILKYILLG SN*

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

6 entries for 6 sites

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SiteModificationModifierReference
K2ubiquitinylated lysineSwaney DL, et al. (2013) PMID: 23749301
T72phosphorylated residueBreitkreutz A, et al. (2010) PMID: 20489023
S73phosphorylated residueBreitkreutz A, et al. (2010) PMID: 20489023
T81phosphorylated residueBreitkreutz A, et al. (2010) PMID: 20489023
K172ubiquitinylated lysineSwaney DL, et al. (2013) PMID: 23749301
K276ubiquitinylated lysineSwaney DL, et al. (2013) PMID: 23749301
Showing 1 to 6 of 6 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A103.11
C41.24
D268.07
E154.66
F113.42
G154.66
H41.24
I278.39
K195.90
L4213.04
M72.17
N144.35
P103.11
Q144.35
R175.28
S278.39
T195.90
V299.01
W10.31
Y113.42

Physical Details

Length (a.a): 322
Molecular Weight (Da): 36396.4
Isoelectric Point (pl): 5.46
Formula: C1627H2632N429O490S11
Aliphatic Index: 108.52
Instability Index: 32.15

Coding Region Translation Calculations

Codon Bias: 0.13
Codon Adaptation Index: 0.16
Frequence of Optimal Codons: 0.5
Hydropathicity of Protein: -0.01
Aromaticity Score: 0.07

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 22140.0
NO Cys residues appear as half cystines: 21890.0

Atomic Composition

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Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

45 entries for 11 sources

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External IDSource
AN10854AspGD
orf19.5768CGD
592DIP
356624594GenBank/EMBL/DDBJ
6321323GenBank/EMBL/DDBJ
D16506GenBank/EMBL/DDBJ
CAA96823.1GenBank/EMBL/DDBJ
BAA03958.1GenBank/EMBL/DDBJ
AAA35061.1GenBank/EMBL/DDBJ
AAA34472.1GenBank/EMBL/DDBJ
Showing 1 to 10 of 45 entries

Resources

Homologs

AGD | AnalogYeast | AspGD | BLASTP at NCBI | CGD | FungiDB | PhylomeDB | PomBase | YGOB | YOGY

Protein Databases

AlphaFold Protein Structure | GPMDB | ModelArchive | Pfam domains | SUPERFAMILY | TopologYeast | UniProtKB

Localization

CYCLoPs | dHITS | LoQAtE | YeastGFP | YeastRC Public Images | YeastRGB | YPL+

Post-translational Modifications

CoSMoS.c. | PhosphoGRID | PhosphoPep