SPT6 / YGR116W Overview
- Standard Name
- SPT6 1
- Systematic Name
- YGR116W
- SGD ID
- SGD:S000003348
- Aliases
- CRE2 9 , SSN20 10
- Feature Type
- ORF , Verified
- Description
- Histone chaperone; regulates chromatin structure, transcription, mRNA 3'-end formation and genome stability; role in chromatin remodeling and nucleosome assembly; required for initiation of DNA replication, promoting origin licensing through MCM helicase loading; required for fidelity of promoter selection through maintenance of chromatin structure during transcription; required for H3K36 trimethylation but not dimethylation by Set2p 2 3 4 5 6 7 8
- Name Description
- SuPpressor of Ty's 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Spt6p is 1451 amino acids long, of average half-life, moderate in abundance; ubiquitinylated on K1355, acetylated on 4 lysines, phosphorylated on 18 residues
- Length (a.a.)
- 1451
- Mol. Weight (Da)
- 168205.4
- Isoelectric Point
- 4.63
- Median Abundance (molecules/cell)
- 8837 +/- 2877
- Half-life (hr)
- 9.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all SPT6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-binding RNA polymerase II transcription factor involved in nucleosome remodeling, transcription antitermination, and mRNA export from the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables histone binding (IDA)
- enables nucleosome binding (IMP, IDA)
- enables transcription antitermination factor activity, DNA binding (IDA, IMP, IGI)
Biological Process
- Manually Curated
- involved in cellular response to stress (IMP)
- involved in chromatin organization (IMP)
- involved in chromatin remodeling (IGI, IMP)
- involved in G1/S transition of mitotic cell cycle (IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP)
- involved in nucleosome assembly (IDA)
- involved in nucleosome organization (IMP)
- involved in poly(A)+ mRNA export from nucleus (IMP)
- acts upstream of or within positive regulation of DNA replication origin binding (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP)
- involved in positive regulation of transcription elongation by RNA polymerase II (IDA)
- involved in regulation of mRNA 3'-end processing (IGI, IMP)
- involved in regulation of transcription by RNA polymerase II (IMP)
- involved in regulation of transcriptional start site selection at RNA polymerase II promoter (IMP)
- involved in transcription antitermination (IGI, IMP)
Cellular Component
- Manually Curated
- located in euchromatin (IDA)
- located in mitochondrion (HDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene in strain S288C, non-essential gene in strain Sigma1278b, in which nulls cannot grow invasively; conditional mutants glow slowly, are sensitive to heat; overexpression slows growth
Classical Genetics
- null
- reduction of function
- conditional
- null
- overexpression
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Spt6p interacts physically with proteins involved in transcription; SPT6 interacts genetically with genes involved in transcription
345 total interactions for 218 unique genes
Physical Interactions
- Affinity Capture-MS: 101
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 29
- Biochemical Activity: 4
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 5
- Far Western: 2
- Protein-peptide: 4
- Proximity Label-MS: 3
- Reconstituted Complex: 10
- Two-hybrid: 3
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 3
- Negative Genetic: 78
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 7
- Positive Genetic: 14
- Synthetic Growth Defect: 27
- Synthetic Haploinsufficiency: 2
- Synthetic Lethality: 19
- Synthetic Rescue: 21
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- Spt6p is a highly-conserved histone chaperone and chromatin-remodeling factor that plays a number of distinct chromatin-dependent and chromatin-independent roles in transcription and mRNA processing. Spt6 is a large 168-kDa protein with an unstructured, acidic N-terminus and an SH2 fold in its C-terminal domain. Spt6p forms a complex with Spn1p that functions in the transition of poised promoters to active states of RNA polymerase II (RNAPII) elongation. RNAPII and the transcription factor Spn1p occupy the poised promoter in the uninduced state, while Spt6p and the Swi/Snf complex are recruited upon activation. Spt6p promotes reassembly of nucleosomes following passage of RNA polymerase II. Spt6p binds nucleosomes directly, and this interaction is negatively regulated by Spn1p. Spt6p also functions in mRNA processing and export, and is required for proper 3'-end formation by preventing premature 3'-end processing at upstream polyadenylation signals. Spt6p has also been implicated in mRNA splicing.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-06-24
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.