BUB1 / YGR188C Overview
- Standard Name
- BUB1 1
- Systematic Name
- YGR188C
- SGD ID
- SGD:S000003420
- Feature Type
- ORF , Verified
- Description
- Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication 2 3 4 5 6 7 8
- Name Description
- Budding Uninhibited by Benzimidazole 1
- Paralog
- MAD3 8
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- BUB1 has a paralog, MAD3, that arose from whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 1021
- Mol. Weight (Da)
- 117861.4
- Isoelectric Point
- 6.69
- Median Abundance (molecules/cell)
- 414 +/- 412
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all BUB1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein kinase involved in mitotic spindle assembly checkpoint, centromere complex assembly; localizes to the kinetochore
View computational annotations
Molecular Function
- Manually Curated
- enables protein binding (IPI)
- enables protein kinase activity (IDA)
Biological Process
- Manually Curated
- involved in centromere complex assembly (IMP)
- involved in macroautophagy (IMP)
- involved in mitotic spindle assembly checkpoint signaling (IMP)
- involved in protein localization to kinetochore (IMP)
- involved in sister chromatid biorientation (IMP)
Cellular Component
- Manually Curated
- located in condensed chromosome, centromeric region (IDA)
- located in kinetochore (IDA)
- located in nucleus (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is defective in cell cycle progression through the G2/M phase transition and shows increased chromosome instability; mutations confer sensitivity to multiple chemicals, including benomyl, nocodazole, cycloheximide
Classical Genetics
- null
- autophagy: decreased
- cell cycle progression through the G2/M phase transition: abnormal
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- colony sectoring: increased
- mitophagy: decreased
- protein activity: decreased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- spindle morphology: abnormal
- viability: decreased
- reduction of function
- activation
- null
- acid pH resistance: decreased
- budding index: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- lipid particle morphology: abnormal
- mitotic recombination: decreased
- oxidative stress resistance: increased
- resistance to chemicals: decreased
- respiratory growth: decreased rate
- toxin resistance: decreased
- transposable element transposition: decreased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The bub1 null mutant is viable; the null mutant of paralog mad3 is viable; the bub1 mad3 double mutant is viable.
1125 total interactions for 730 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 16
- Biochemical Activity: 4
- Co-crystal Structure: 1
- Co-localization: 5
- Reconstituted Complex: 6
- Two-hybrid: 14
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 2
- Dosage Rescue: 9
- Negative Genetic: 609
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 12
- Positive Genetic: 177
- Synthetic Growth Defect: 28
- Synthetic Lethality: 204
- Synthetic Rescue: 14
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2000-03-31
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.