Phenotype Help

TDH3 / YGR192C Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant is temperature sensitive, has a decreased growth rate and reduced competitive fitness in various growth media; null mutant displays increased survival following a ramped heat stimulus; null mutant has decreased desiccation resistance, and acid pH resistance but increased resistance to zinc deficiency; haploinsufficiency results in chromosome instability; overexpression results in a decreased growth rate, and an extended G1 phase

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

42 entries for 20 phenotypes


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
acid pH resistance: decreased
systematic mutation setnull
Allele: tdh3-Δ
S288C0.1-0.4% boric acidSchmidt M, et al. (2012) PMID:22902726
cell cycle progression: abnormal
large-scale surveyoverexpressionW303Details: greater than 3% shift in cells containing a 1C DNA content, indicative of a G1 phase arrest or delay
Stevenson LF, et al. (2001) PMID:11274415
cell size: increased
classical genetics null
Allele: tdh3-Δ
S288CDetails: increased cell size in asynchronous cultures, and the null mutant has a larger birth size
Blank HM, et al. (2023) PMID:37792491
chemical compound accumulation: decreased
systematic mutation setnull
Allele: tdh3-Δ
S288C calcium ionYu D, et al. (2012) PMID:23151179
chemical compound accumulation: decreased
systematic mutation setnull
Allele: tdh3-Δ
S288C hydrogen sulfideMedia: cysteine supplementation, 500 mg/L
Winter G, et al. (2014) PMID:25517415
chromosome/plasmid maintenance: decreased
heterozygous diploid, systematic mutation setnull
Allele: tdh3-Δ
S288CAssay: diploid bimater (BiM) assay
Details: haploinsufficiency results in chromosome instability (CIN); scored >= 2 standard deviations above the mean
Choy JS, et al. (2013) PMID:23825022
colony sectoring: increased
heterozygous diploid, systematic mutation setnull
Allele: tdh3-Δ
S288CAssay: quantitative chromosome transmission fidelity (qCTF) assay
Details: >1.5 fold increase in mini-chromosome loss relative to wt, indicative of chromosome instability (CIN)
Zhu J, et al. (2015) PMID:25823586
competitive fitness: decreased
homozygous diploid, competitive growth

genome-wide fitness profiling

null
Allele: tdh3-Δ
S288CMedia: YPD
Details: Relative fitness score: 0.971
Deutschbauer AM, et al. (2005) PMID:15716499
competitive fitness: decreased
competitive growth

fitness profiling using complete deletion alleles

null
Allele: tdh3-Δ
S288CMedia: minimal medium
Details: Relative fitness score: 0.943
Breslow DK, et al. (2008) PMID:18622397
competitive fitness: decreased
systematic mutation setnull
Allele: tdh3-Δ
S288CMedia: rich medium (YPD) w/6% ethanol, ETH
Qian W, et al. (2012) PMID:23103169
Showing 1 to 10 of 42 entries

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).


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