Phenotype Help

ARG2 / YJL071W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.


Summary
Non-essential gene; null mutant is auxotrophic for arginine and is unable to use allantoin as nitrogen source; null mutant has decreased competitive fitness in synthetic media but increased fitness in media containing glycerol or ethanol; null has decreased resistance to alpha-synuclein expression, and accumulates increased reactive oxygen species (ROS), while overexpression decreases the accumulation of ROS associated associated with alpha-synuclein expression; systematic null mutant has decreased telomere length and increased metal reductase activity

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

20 entries for 16 phenotypes


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PhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetailsReference
auxotrophy
classical geneticsnull
Allele: arg2-Δ
S288C arginineVandenbol M and Portetelle D (1999) PMID:10509023
chemical compound accumulation: decreased
classical geneticsoverexpressionOther reactive oxygen speciesTreatment: alpha-synuclein expressed
Details: ~50% reduction in ROS signal compared with wild-type
Liang J, et al. (2008) PMID:18772193
chemical compound accumulation: increased
classical geneticsnull
Allele: arg2-Δ
Other reactive oxygen speciesTreatment: alpha-synuclein expressed
Details: 32% increase in ROS signal compared with wild-type
Liang J, et al. (2008) PMID:18772193
competitive fitness: decreased
systematic mutation set

fitness profiling after 15 generations

null
Allele: arg2-Δ
S288CMedia: synthetic medium, minimal +his/leu/ura medium
Giaever G, et al. (2002) PMID:12140549
competitive fitness: increased
systematic mutation setnull
Allele: arg2-Δ
S288C ethanolQian W, et al. (2012) PMID:23103169
competitive fitness: increased
systematic mutation setnull
Allele: arg2-Δ
S288CMedia: glycerol medium, YPG
Qian W, et al. (2012) PMID:23103169
competitive fitness: increased
systematic mutation setnull
Allele: arg2-Δ
S288CMedia: rich medium (YPD) w/6% ethanol, ETH
Qian W, et al. (2012) PMID:23103169
mitotic recombination: decreased
systematic mutation set null
Allele: arg2-Δ
S288CDetails: spontaneous direct-repeat hypo-recombination identified using a high-throughput replica-pinning technique; recombination frequency of less than 33% compared to 56% for wt
Novarina D, et al. (2020) PMID:32265288
protein activity: increased
Reporter: metal reductase
systematic mutation set

cell surface metal reductase activity quantified using BPS and scoring colonies for red color

null
Allele: arg2-Δ
S288CDong K, et al. (2013) PMID:23959798
resistance to chemicals: decreased
homozygous diploid, competitive growth null
Allele: arg2-Δ
S288C674.9 μM N-nitrosodimethylamineDetails: 2-fold or greater sensitivity to NDMA treatment based on a fitness defect (FD) score significance threshold of 1.0, expressed as the log2 ratio of a mutant strains fitness relative to control in a chemogenomic screen
Ogbede JU, et al. (2021) PMID:34127714
Showing 1 to 10 of 20 entries

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