GSH1 / YJL101C Overview
- Standard Name
- GSH1 1
- Systematic Name
- YJL101C
- SGD ID
- SGD:S000003637
- Feature Type
- ORF , Verified
- Description
- Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress 1 2 3 4 5 6
- Name Description
- glutathione (GSH) 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- GSH1 is located in the middle of the left arm of chromosome X between tRNA-Arg and LSB6 phosphatidylinositol 4-kinase; coding sequence is 2037 nucleotides long with 17 SNPs, 5 of which lead to amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Gsh1p is 678 amino acids long, shorter-lived, low in abundance; acetylated on K337, ubiquitinylated on K677, phosphorylated on 4 residues; protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 678
- Mol. Weight (Da)
- 78241.2
- Isoelectric Point
- 6.22
- Median Abundance (molecules/cell)
- 3495 +/- 1065
- Half-life (hr)
- 8.3
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all GSH1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Gamma-glutamylcysteine synthetase responsible for the first step of glutathione biosynthesis; catalyzes the conversion of cysteine and glutamate to gamma-glutamylcysteine, consuming ATP; also involved in the response to hydrogen peroxide and cadmium
View computational annotations
Molecular Function
- Manually Curated
- enables glutamate-cysteine ligase activity (IDA, IMP)
Biological Process
- Manually Curated
- involved in glutathione biosynthetic process (IMP, IDA)
- involved in response to cadmium ion (IDA)
- involved in response to hydrogen peroxide (IMP)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation confers glutathione auxotrophy, slow growth and increased sensitivity to oxidative stress
Classical Genetics
- null
- auxotrophy
- budding index: decreased
- cell death: increased
- cell size: increased
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chromosome/plasmid maintenance: abnormal
- chronological lifespan: decreased
- colony color: abnormal
- growth in exponential phase: decreased rate
- metal resistance: decreased
- oxidative stress resistance: decreased
- protein activity: increased
- protein/peptide modification: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- respiratory metabolism: decreased
- RNA accumulation: decreased
- vegetative growth: decreased
- viability: increased
- overexpression
- null
- chemical compound accumulation: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- metal resistance: decreased
- mitophagy: increased
- oxidative stress resistance: decreased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: absent
- respiratory growth: decreased
- stress resistance: decreased
- utilization of carbon source: increased
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Gsh1p interacts physically with proteins involved in RNA catabolism, translation regulation, and nucleobase transport; GSH1 interacts genetically with genes involved in transcription
623 total interactions for 471 unique genes
Physical Interactions
- Affinity Capture-MS: 10
- Affinity Capture-RNA: 5
- Proximity Label-MS: 1
- Reconstituted Complex: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 3
- Negative Genetic: 446
- Phenotypic Enhancement: 6
- Phenotypic Suppression: 9
- Positive Genetic: 45
- Synthetic Growth Defect: 37
- Synthetic Lethality: 11
- Synthetic Rescue: 48
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 15
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-01-02
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.