RSF2 / YJR127C Overview
- Standard Name
- RSF2 1
- Systematic Name
- YJR127C
- SGD ID
- SGD:S000003888
- Aliases
- ZMS1 2
- Feature Type
- ORF , Verified
- Description
- Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress 1 2 3 4 5 6
- Name Description
- ReSpiration Factor 1
- Paralog
- TDA9 5
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- RSF2 has a paralog, TDA9, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Relocalizes from nucleus to cytoplasm upon DNA replication stress
- Length (a.a.)
- 1380
- Mol. Weight (Da)
- 155072.0
- Isoelectric Point
- 8.07
- Median Abundance (molecules/cell)
- 1222 +/- 522
- Half-life (hr)
- 2.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RSF2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding protein involved in transcription regulation; localizes to both nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables sequence-specific DNA binding (HDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in regulation of DNA-templated transcription (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- null
- acid pH resistance: decreased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- competitive fitness: increased
- haploinsufficient
- resistance to chemicals: decreased
- resistance to chemicals: increased
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The rsf2 null mutant is viable; the null mutant of paralog tda9 is viable; the rsf2 tda9 double mutant displays negative genetic interactions.
72 total interactions for 67 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 10
- Co-purification: 1
- PCA: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Rescue: 1
- Negative Genetic: 41
- Positive Genetic: 5
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- RFS2 encodes a putative transcription factor that is a member of the C2H2 zinc finger class. Rsf2p was shown to bind DNA in a large-scale study. The rsf2 null mutant has a mild growth defect on glycerol and lactate media, and microarray experiments show that the transcription of about 100 genes is affected both positively and negatively. The null mutant displays reduced transcription of some genes involved in respiratory growth, such as GUT1 and GUT2, respectively encoding glycerol kinase and glycerol-3-phosphate dehydrogenase; COX4, encoding a subunit of cytochrome c oxidase; MRP13, encoding a mitochondrial ribosomal subunit; and RPO41, encoding mitochondrial RNA polymerase. Overexpression of Rsf2p increases levels of the ALD6 mRNA.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.