CCW12 / YLR110C Overview
- Standard Name
- CCW12 1
- Systematic Name
- YLR110C
- SGD ID
- SGD:S000004100
- Feature Type
- ORF , Verified
- Description
- Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication 1 2 3 4
- Name Description
- Covalently linked Cell Wall protein 1
- Paralog
- CCW22 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 133
- Mol. Weight (Da)
- 13052.7
- Isoelectric Point
- 4.29
- Median Abundance (molecules/cell)
- 13881 +/- 12238
- Half-life (min)
- 10.0
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CCW12 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Structural constituent of cell wall; involved in agglutination during conjugation and fungal cell wall organization; localizes to the bud tip and mating projection tip
View computational annotations
Molecular Function
- Manually Curated
- enables structural constituent of cell wall (IMP)
Biological Process
- Manually Curated
- involved in agglutination involved in conjugation with cellular fusion (IMP)
- involved in conjugation with cellular fusion (IMP)
- involved in fungal-type cell wall organization (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in cellular bud tip (IDA)
- is active in fungal-type cell wall (IDA)
- located in fungal-type vacuole (HDA)
- located in mating projection tip (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutation causes abnormalities in bud and mating projection (shmoo) morphology, increased chitin deposition in cell wall, higher sensitivity to hypoosmotic stress and to cell wall-affecting chemicals (Congo Red, Calcofluor White); both null mutation and overexpression result in slow growth
Classical Genetics
- null
- bud morphology: abnormal
- bud neck morphology: abnormal
- budding: abnormal
- cell shape: abnormal
- cell size: increased
- cell wall morphology: abnormal
- chitin deposition: increased
- hypoosmotic stress resistance: decreased
- mating efficiency: decreased
- mating projection morphology: abnormal
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- survival rate in stationary phase: decreased
- vegetative growth: decreased
- vegetative growth: decreased rate
- overexpression
- null
- biofilm formation: increased
- bud morphology: abnormal
- budding pattern: abnormal
- cell shape: abnormal
- chemical compound accumulation: abnormal
- chitin deposition: increased
- chronological lifespan: decreased
- chronological lifespan: increased
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: decreased
- heat sensitivity: increased
- killer toxin resistance: increased
- mitotic recombination: decreased
- protein transport: abnormal
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: increased rate
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased rate
- viable
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The ccw12 null mutant is viable; the null mutant of paralog ccw22 is viable; the ccw12 ccw22 double mutant has not been annotated for phenotype.
502 total interactions for 315 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 15
- Co-purification: 1
- PCA: 4
- Reconstituted Complex: 1
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Dosage Rescue: 5
- Negative Genetic: 276
- Phenotypic Enhancement: 30
- Phenotypic Suppression: 43
- Positive Genetic: 45
- Synthetic Growth Defect: 39
- Synthetic Lethality: 28
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 23
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.