SWI6 / YLR182W Overview

Standard Name
SWI6 1
Systematic Name
PSL8 , SDS11 8
Feature Type
ORF , Verified
Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators 2 3 4 5 6 7
Name Description
SWItching deficient 1
Comparative Info
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Median Abundance (molecules/cell)
3241 +/- 1240
Half-life (hr)


Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.

View all SWI6 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Transcription cofactor that activates transcription by RNA polymerase II during heat stress and during the G1/S mitotic transition; also regulates meiotic recombination; component of both the MBF and SBF complexes

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated


Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.

Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; mull mutant grows slowly, has large abnormally-shaped cells, cannot utilize various nitrogen sources, is impaired in fermentation, sensitive to caffeine, heat, cold, alcohols, metals, DNA-damaging agents, various antibiotics, antimalarial quinine, and oxidative stress; cell cycle progression is delayed at G1 and the G2/M transition; overexpression slows growth
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

1098 total interactions for 748 unique genes

Physical Interactions

  • Affinity Capture-MS: 81
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 39
  • Biochemical Activity: 10
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 6
  • Protein-peptide: 1
  • Proximity Label-MS: 2
  • Reconstituted Complex: 10
  • Two-hybrid: 8

Genetic Interactions

  • Dosage Growth Defect: 6
  • Dosage Rescue: 23
  • Negative Genetic: 466
  • Phenotypic Enhancement: 12
  • Phenotypic Suppression: 25
  • Positive Genetic: 235
  • Synthetic Growth Defect: 83
  • Synthetic Lethality: 69
  • Synthetic Rescue: 10
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

SWI6 encodes a transcription cofactor that regulates transcription of the G1/S gene cluster in the cell cycle as part of heterodimers formed with two different DNA-binding transcription factors, Swi4p and Mbp1p. With Swi4p, Swi6p forms the SBF (Swi4p/Swi6p cell-cycle box (SCB) binding factor) complex, whose targets include G1 cyclins (CLN1, CLN2, PCL1, PCL2) and the HO endonuclease. With Mbp1p, Swi6p forms the MBF (Mlu1 cell cycle box (MCB) Binding Factor) complex. MBF targets partially overlap with those of SBF, and include genes involved in DNA synthesis and DNA repair (for example, CDC21, CDC8, and CDC9, and G1 cyclins, as well as SWI4). SBF and MBF both repress G1/S genes during early G1 phase. Whi5p binding to the SBF complex in early G1 causes SBF to repress transcription. After phosphorylation of Whi5p by Cdc28p/Cln3p it dissociates from SBF, allowing SBF to activate transcription. Cdc28p/Cln3p also activates MBF-regulated promoters. Transcription of the CLN1 and CLN2 genes by SBF feeds back to further activate both SBF- and MBF-regulated promoters. Swi6p and SBF are also involved in chromatin reorganization at certain promoters such as CLN2 and HO via recruitment of the FACT complex. One target of MBF activation is Nrm1p, which binds to Swi6p at MBF target promoters and promotes the repressor activity of MBF. Increasing Cdc28p/Clb2p activity in late G1 phase promotes the repressor activity of both SBF and MBF. Phosphorylation of Swi6p by Cdc28p/Clb6p results in the accumulation of Swi6p in the cytoplasm in late G1, also helping to shut down transcription of the G1/S gene cluster as cells exit the G1 phase.
Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.