MET17 / YLR303W Overview
- Standard Name
- MET17 1
- Systematic Name
- YLR303W
- SGD ID
- SGD:S000004294
- Aliases
- MET15 27 , MET25 28
- Feature Type
- ORF , Verified
- Description
- O-acetyl homoserine-O-acetyl serine sulfhydrylase; involved in methionine and cysteine biosynthesis; long considered essential for inorganic sulfur assimilation, but inefficient homocysteine synthase Hsu1p enables met17 null cells to assimilate enough inorganic sulfur for survival and proliferation; growth failure of met17 null can be explained by toxic accumulation of H2S gas due to a metabolic bottleneck 2 3 4
- Name Description
- METhionine requiring 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- MET17 is located on the right arm of chromosome XII between uncharacterized ORF YLR302C and YLRCdelta19 Ty1 LTR; coding sequence is 363 nucleotides long with 4 SNPs, one of which causes a Val/Ile amino acid polymorphism at residue 105
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Met17p is 444 amino acids long, very long-lived, high in abundance; acetylated on 6 lysines, ubiquitinylated on K160 and K412, succinylated on K231, phosphorylated on 17 residues
- Length (a.a.)
- 444
- Mol. Weight (Da)
- 48663.0
- Isoelectric Point
- 6.41
- Median Abundance (molecules/cell)
- 38332 +/- 13719
- Half-life (hr)
- 34.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MET17 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Bifunctional enzyme with O-acetylserine and O-acetylhomoserine sulfhydrylase activities involved in methionine and cysteine metabolism; catalyses the reaction between acetylated serine or homoserine with thiol to produce the corresponding amino acids; localized to the cytoplasm and plasma membrane
View computational annotations
Molecular Function
- Manually Curated
- enables cysteine synthase activity (IDA)
- enables O-acetylhomoserine sulfhydrylase activity (IDA)
Biological Process
- Manually Curated
- involved in cysteine biosynthetic process (TAS)
- involved in methionine metabolic process (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- located in plasma membrane (HDA)
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is auxotrophic for methionine, grows slowly, shows increased oxidative stress resistance, increased metal resistance, decreased competitive fitness, increased chronological lifespan, increased H2S production
Classical Genetics
- null
- auxotrophy
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: increased
- metal resistance: increased
- protein/peptide accumulation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- vegetative growth: decreased
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Met17p interacts physically with proteins involved in translation; MET17 interacts genetically with genes involved in DNA repair
131 total interactions for 120 unique genes
Physical Interactions
- Affinity Capture-MS: 42
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 1
- Biochemical Activity: 5
- Co-purification: 1
- Proximity Label-MS: 3
Genetic Interactions
- Dosage Rescue: 4
- Negative Genetic: 50
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 1
- Positive Genetic: 7
- Synthetic Growth Defect: 6
- Synthetic Lethality: 3
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 18
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2011-02-07
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.