RSC2 / YLR357W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

RSC2/YLR357W is a non-essential gene in reference strain S288C, null mutants are viable but show broad defects in growth, stress tolerance, and genome maintenance. Null and conditional alleles confer heat sensitivity (33–37°C), impaired respiration (absent growth on ethanol at 37°C and poor growth on glycerol), slower fermentative growth, and reduced competitive fitness across rich and synthetic media. Cells display abnormal chromosome/plasmid maintenance, shortened telomeres, increased transcriptional silencing, and altered cell biology, including enlarged cells and nucleoli, abnormal buds and vacuoles (exacerbated by high salt), increased RNA and glycogen accumulation, higher reactive oxygen species with SDS, elevated glutathione excretion, abnormal protein modification and distribution, and increased protein secretion. Deletion strains are myo‑inositol auxotrophs, have decreased chronological lifespan, and altered colony appearance; they also use galactose and nitrogen sources (allantoin, proline) more slowly. rsc2Δ is broadly hypersensitive to many chemicals and stresses (DNA damage, translation/transcription inhibitors, cell wall/membrane stressors, solvents, and several metals), yet shows increased resistance to select agents (e.g., tunicamycin, camptothecin, quinine, palmitoleic acid, mycophenolic acid, 2,4‑diacetylphloroglucinol, nickel sulfate, yttrium chloride), with cycloheximide resistance varying by assay. Killer toxin resistance is reduced, but resistance to in vivo EcoRI expression is increased. Overexpression decreases growth and fitness and enhances invasive growth in Sigma1278b; missense alleles D461G and V457M also cause heat sensitivity.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

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