STE11 / YLR362W Overview
- Standard Name
- STE11 1
- Systematic Name
- YLR362W
- SGD ID
- SGD:S000004354
- Feature Type
- ORF , Verified
- Description
- Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress 2 3 4 5 6
- Name Description
- STErile 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 717
- Mol. Weight (Da)
- 80718.8
- Isoelectric Point
- 7.21
- Median Abundance (molecules/cell)
- 1533 +/- 425
- Half-life (hr)
- 7.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all STE11 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- MAP kinase kinase kinase that is involved in signal transduction during pseudohyphal growth, hyperosmotic response, filamentous growth, mating in response to pheromone, and the cell integrity pathway; also involved in regulation of RNA-mediated transposition and the cellular response to heat; localizes to the cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables MAP kinase kinase kinase activity (IDA)
- enables protein kinase activity (HDA)
- enables SAM domain binding (IDA)
Biological Process
- Manually Curated
- involved in cell integrity MAPK cascade (IGI)
- involved in cellular hyperosmotic response (IMP, IGI)
- involved in invasive growth in response to glucose limitation (IMP)
- involved in osmosensory signaling pathway via Sho1 osmosensor (IGI)
- involved in p38MAPK cascade (IMP, IGI)
- involved in pheromone response MAPK cascade (IDA, IGI, IMP)
- involved in pheromone-dependent signal transduction involved in conjugation with cellular fusion (IMP)
- involved in pseudohyphal growth (IMP)
- involved in signal transduction involved in filamentous growth (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; conditional mutant is pheromone resistant, being unable to undergo pheromone-induced cell cycle arrest or form shmoos, unable to produce mating pheromone Mfa1p/2p and is sterile; null mutant has strong pseudohyphal and invasive growth defects, is sensitive to hyperosmotic stress and hypersensitive to enzymatic treatment with zymolyase; constitutively active mutant has enhanced pseudohyphal growth, a vegetative growth defect and displays activation-related phosphorylation of MAPKs Fus3p and Kss1p in the absence of mating pheromone; heterozygous diploid null is haploinsufficient
Classical Genetics
- null
- cell death: absent
- colony appearance: abnormal
- filamentous growth: decreased
- hyperosmotic stress resistance: decreased
- invasive growth: decreased
- mating response: absent
- metal resistance: decreased
- osmotic stress resistance: decreased
- pheromone-induced cell cycle arrest: absent
- protein/peptide modification: absent
- pseudohyphal growth: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- toxin resistance: increased
- conditional
- activation
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- haploinsufficient
- killer toxin resistance: decreased
- liquid culture appearance: abnormal
- mating response: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: absent
- viable
- reduction of function
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
298 total interactions for 130 unique genes
Physical Interactions
- Affinity Capture-MS: 25
- Affinity Capture-RNA: 4
- Affinity Capture-Western: 34
- Biochemical Activity: 11
- Co-crystal Structure: 3
- Co-fractionation: 2
- Co-purification: 2
- FRET: 6
- PCA: 11
- Proximity Label-MS: 1
- Reconstituted Complex: 34
- Two-hybrid: 42
Genetic Interactions
- Dosage Rescue: 16
- Negative Genetic: 1
- Phenotypic Enhancement: 20
- Phenotypic Suppression: 56
- Synthetic Growth Defect: 9
- Synthetic Lethality: 7
- Synthetic Rescue: 14
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2007-03-06
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.