MAG2 / YLR427W Overview
- Standard Name
- MAG2 1
- Systematic Name
- YLR427W
- SGD ID
- SGD:S000004419
- Feature Type
- ORF , Verified
- Description
- Cytoplasmic RING-finger type E3 ubiquitin ligase; involved in monoubiquitination of Rps3p, a 40S subunit from decoding-defective ribosomes, leading to Fap1p-mediated K63-linked polyubiquitination, followed by dissociation of non-functional 80S ribosomes and non-functional rRNA decay (NRD) of 18S rRNA; ubiquitinated; induced in response to mycotoxin patulin; predicted to be involved in repair of alkylated DNA due to interaction with MAG1; similar to the human ring finger motif protein RNF10 1 2 3 4 5 6
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Mag2p is 670 amino acids long, slightly shorter-lived, low in abundance; contains 1 zinc finger; phosphorylated on S634, ubiquitinylated on 4 lysines
- Length (a.a.)
- 670
- Mol. Weight (Da)
- 76328.4
- Isoelectric Point
- 8.77
- Median Abundance (molecules/cell)
- 1601 +/- 622
- Half-life (hr)
- 9.2
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all MAG2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic zinc-binding ubiquitin E3 ligase involved in protein monoubiquitination during detection of stalled ribosomes for subsequent nonfunctional rRNA decay
View computational annotations
Molecular Function
- Manually Curated
- enables ubiquitin protein ligase activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in nonfunctional rRNA decay (IDA)
- involved in protein monoubiquitination (IDA)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- is active in cytosolic ribosome (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null displays small defect in vacuolar fragmentation, decreased resistance to the toxicity of Aβ42, decreased competitive fitness, decreased resistance to indium, increased resistance to toxaphene, decreased desiccation resistance; overexpression slows growth
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Mag2p interacts physically with proteins involved in rRNA processing and translation regulation; MAG2 interacts genetically with genes invovled in transcription
89 total interactions for 86 unique genes
Physical Interactions
- Affinity Capture-MS: 40
- Affinity Capture-RNA: 5
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 1
- Reconstituted Complex: 1
Genetic Interactions
- Negative Genetic: 30
- Phenotypic Suppression: 1
- Positive Genetic: 8
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.