RAD52 / YML032C Overview
- Standard Name
- RAD52 1
- Systematic Name
- YML032C
- SGD ID
- SGD:S000004494
- Feature Type
- ORF , Verified
- Description
- Protein involved in homologous recombination; attenuates resection of DNA double-strand break ends and stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA (ssDNA); anneals complementary ssDNA; involved in the repair of double-strand breaks in DNA during vegetative growth, meiosis and UV induced sister chromatid recombination; forms a homodecameric ring; phosphorylation by Cdc28p in G2/M phase promotes Rad52p ring interaction that leads to superstructure formation 2 3 4 5 6 7 8 9
- Name Description
- RADiation sensitive 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rad52p is 471 amino acids long, shorter-lived, low in abundance; phosphorylated on 13 residues, sumoylated on 13 residues
- Length (a.a.)
- 471
- Mol. Weight (Da)
- 52406.3
- Isoelectric Point
- 8.56
- Median Abundance (molecules/cell)
- 2164 +/- 343
- Half-life (hr)
- 6.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RAD52 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Recombinase involved in the repair of DNA double-strand breaks and single-strand postreplication gaps, as well as the formation of meiotic four-stranded branched intermediates
View computational annotations
Molecular Function
- Manually Curated
- enables DNA strand exchange activity (IDA)
- enables DNA/DNA annealing activity (IDA)
Biological Process
- Manually Curated
- involved in DNA amplification (IMP)
- involved in DNA recombinase assembly (IDA)
- involved in double-strand break repair via break-induced replication (IMP)
- involved in double-strand break repair via homologous recombination (IMP)
- involved in double-strand break repair via single-strand annealing (IGI)
- involved in meiotic joint molecule formation (IMP, IGI)
- involved in mitochondrial DNA repair (IMP)
- involved in postreplication repair (IMP)
- involved in telomere maintenance via recombination (IMP)
Cellular Component
- Manually Curated
- located in nuclear chromosome (IDA)
- located in nucleus (IDA, HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to DNA damaging agents, DNA synthesis and topoisomerase inhibitors; null mutant is sensitive to X-rays, gamma rays and UV radiation; null displays increased chromosome instability including gross chromosomal rearrangements, increased mutation rate, elevated Ty element transposition and decreased mitotic and ectopic recombination in meiosis; null mutant is temperature sensitive, and has decreased growth rate and competitive fitness on rich and synthetic media; null mutant has decreased replicative lifespan, increased desiccation sensitivity and decreased resistance to zinc deficiency; null contains large-budded cells with nuclear extensions at the nuclear envelope, adjacent to the nucleolus; homozygous reduction of function mutant has a sporulation defect, and the heterozygous null mutant is haploinsufficient
Classical Genetics
- null
- bud morphology: abnormal
- cell cycle progression in G2 phase: increased duration
- cellular morphology: abnormal
- chromosome/plasmid maintenance: abnormal
- chromosome/plasmid maintenance: decreased
- chronological lifespan: decreased
- colony sectoring: increased
- endoplasmic reticulum morphology: abnormal
- gamma ray resistance: decreased
- growth in exponential phase: decreased rate
- ionizing radiation resistance: decreased
- meiotic recombination: decreased
- metal resistance: decreased
- metal resistance: normal
- mitotic recombination: decreased
- mutation frequency: decreased
- mutation frequency: increased
- nuclear morphology: abnormal
- oxidative stress resistance: decreased
- radiation resistance: decreased
- replicative lifespan: decreased
- resistance to chemicals: absent
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory metabolism: decreased
- toxin resistance: decreased
- transposable element transposition: increased
- UV resistance: decreased
- viability: decreased
- reduction of function
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endoplasmic reticulum morphology: abnormal
- fermentative growth: decreased rate
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: increased
- mitochondrial genome maintenance: abnormal
- mitotic recombination: decreased
- mutation frequency: increased
- nuclear morphology: abnormal
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased
- stress resistance: decreased
- toxin resistance: decreased
- transposable element transposition: decreased
- transposable element transposition: increased
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- unspecified
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rad52p interacts physically with proteins involved in DNA repair; RAD52 interacts genetically with genes involved in DNA repair
1612 total interactions for 596 unique genes
Physical Interactions
- Affinity Capture-MS: 37
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 40
- Biochemical Activity: 6
- Co-crystal Structure: 2
- Co-localization: 8
- Co-purification: 1
- FRET: 1
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 29
- Two-hybrid: 35
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 4
- Dosage Rescue: 19
- Negative Genetic: 473
- Phenotypic Enhancement: 162
- Phenotypic Suppression: 120
- Positive Genetic: 116
- Synthetic Growth Defect: 335
- Synthetic Lethality: 128
- Synthetic Rescue: 84
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2003-01-06
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.