SML1 / YML058W Overview


Standard Name
SML1 1
Systematic Name
YML058W
SGD ID
SGD:S000004523
Feature Type
ORF , Verified
Description
Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication 2 3 4
Name Description
Suppressor of Mec1 Lethality 1
Paralog
DIF1 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
SML1 has a paralog, DIF1, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
104
Mol. Weight (Da)
11829.4
Isoelectric Point
4.45
Median Abundance (molecules/cell)
21089 +/- 9602
Half-life (min)
36.0

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


View all SML1 alleles in SGD search

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Inhibitor of ribonucleoside-diphosphate reductase (RNR); involved in mitochondrion organization and the cellular response to DNA damage; localizes to the nucleus and cytoplasm in high-throughput studies

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant displays increased resistance to DNA damaging agents, while overexpression results in increased sensitivity; null mutant has elevated pools of dNTPs; null mutant has elevated mini-chromosome loss and elevated mitochondrial genome copy number; null mutant displays elevated temperature resistance and increased resistance to fluconazole; null mutant has a pseudohyphal growth defect; heterozygous null mutant is haploinsufficient
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The sml1 null mutant is viable; the null mutant of paralog dif1 is viable; the sml1 dif1 double mutant displays a phenotypic enhancement.

284 total interactions for 162 unique genes

Physical Interactions

  • Affinity Capture-MS: 33
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 4
  • Biochemical Activity: 8
  • Co-crystal Structure: 1
  • Co-localization: 1
  • Co-purification: 1
  • Protein-RNA: 1
  • Reconstituted Complex: 4
  • Two-hybrid: 8

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Lethality: 1
  • Dosage Rescue: 4
  • Negative Genetic: 32
  • Phenotypic Enhancement: 30
  • Phenotypic Suppression: 34
  • Positive Genetic: 6
  • Synthetic Growth Defect: 14
  • Synthetic Lethality: 15
  • Synthetic Rescue: 76
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
10
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
109
Additional
153
Reviews
15

Resources