WAR1 / YML076C Overview
- Standard Name
- WAR1 1
- Systematic Name
- YML076C
- SGD ID
- SGD:S000004541
- Feature Type
- ORF , Verified
- Description
- Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively 1 2
- Name Description
- Weak Acid Resistance 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 944
- Mol. Weight (Da)
- 107562.7
- Isoelectric Point
- 7.64
- Median Abundance (molecules/cell)
- 1383 +/- 321
- Half-life (hr)
- 5.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all WAR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcription factor that activates transcription of organic acid transmembrane transporter PDR12 by RNA polymerase II in response to sorbate
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription factor activity (IMP)
- enables sequence-specific DNA binding (IDA)
Biological Process
- Manually Curated
- involved in cellular response to acidic pH (IDA, IMP)
- involved in positive regulation of transcription by RNA polymerase II (IDA, IMP)
Cellular Component
- Manually Curated
- located in mitochondrion (HDA)
- located in nucleus (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to weak acids and antimalarial quinine; homozygous diploid null sporulates with increased efficiency; overexpression enhances pseudohyphal growth
Classical Genetics
- null
- reduction of function
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
74 total interactions for 72 unique genes
Physical Interactions
- Affinity Capture-MS: 4
- Affinity Capture-RNA: 4
Genetic Interactions
- Negative Genetic: 52
- Positive Genetic: 10
- Synthetic Lethality: 2
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- WAR1 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. War1p activates transcription in response to weak acid stress, and its principal target is PDR12, which encodes a plasma membrane transporter that actively transports weak acids out of the cell. War1p binds constitutively, as a homodimer, to the WARE (weak acid response element) of the PDR12 promoter. Its transcription factor activity is regulated by binding of weak acid anions such as sorbate, benzoate, or propionate, which causes a conformational change that unmasks the transcriptional activation domain. War1p is also regulated by phosphorylation in response to the presence of weak acids.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.