CDC5 / YMR001C Overview
- Standard Name
- CDC5 1
- Systematic Name
- YMR001C
- SGD ID
- SGD:S000004603
- Aliases
- MSD2 20 , PKX2
- Feature Type
- ORF , Verified
- Description
- Polo-like kinase required for mitotic exit; regulates mitotic spindle assembly, nuclear shape, and protein localization to the nucleolus and SPBs; controls targeting and activation of Rho1p at the division site via Rho1p-GEFs; role in adaptation to DNA damage promotes genome instability during replicative senescence; regulates meiotic commitment, spindle assembly, chiasmata formation, recombination intermediate resolution and SC disassembly; human homologs PLK1 and PLK3 complement cdc5 mutants 2 3 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
- Name Description
- Cell Division Cycle 4
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 705
- Mol. Weight (Da)
- 81047.1
- Isoelectric Point
- 9.36
- Median Abundance (molecules/cell)
- 319 +/- 220
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CDC5 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein kinase that plays a role in exit from mitosis; regulates nuclear organization, spindle pole body separation, and protein localization to mitotic spindle pole body; involved in local activation of Rho1p at the division site by activation of GEF, Tus1p; involved in meiotic spindle assembly, synaptonemal complex disassembly, and resolution of meiotic recombination intermediates; associates with the nuclear mitotic cohesin complex and localizes to the nucleus, bud neck, and spindle pole
View computational annotations
Molecular Function
- Manually Curated
- enables centromeric DNA binding (IDA)
- enables phosphoprotein binding (IDA)
- enables protein kinase activity (IDA, IMP, HDA)
- enables protein serine/threonine kinase activity (IDA)
Biological Process
- Manually Curated
- involved in exit from mitosis (IGI)
- involved in G2/M transition of mitotic cell cycle (IPI)
- involved in meiotic spindle assembly (IMP)
- involved in negative regulation of protein localization to nucleolus (IMP, IGI)
- involved in positive regulation of mitotic spindle pole body separation (IGI, IMP)
- involved in positive regulation of protein localization to cell division site involved in mitotic actomyosin contractile ring assembly (IMP)
- involved in positive regulation of Rho protein signal transduction (IMP)
- involved in regulation of nucleus organization (IMP)
- involved in regulation of protein localization to mitotic spindle pole body (IGI)
- acts upstream of or within regulation of protein localization to nucleolus (IMP)
- involved in resolution of meiotic recombination intermediates (IMP)
- involved in synaptonemal complex disassembly (IGI)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA, IMP)
- located in mitotic spindle pole body (IDA)
- located in nucleus (IDA)
- located in spindle pole (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; conditional and repressible mutants arrest as large-budded cells in telophase with an abnormal nuclear morphology; overexpression results in a large multibudded cell cycle arrest with an abnormal actin cytoskeleton, an increase in bipolar budding, and cells with multiple nuclei; homozygous diploid mutants have an increased frequency of chromosome loss, defective meiosis and reduced sporulation
Classical Genetics
- null
- overexpression
- actin cytoskeleton morphology: abnormal
- bipolar budding pattern: increased
- cell cycle progression in G2 phase: abnormal
- cell cycle progression: abnormal
- cell size: increased
- cellular morphology: abnormal
- colony sectoring: increased
- growth in exponential phase: decreased rate
- nuclear morphology: abnormal
- protein/peptide accumulation: increased
- protein/peptide modification: increased
- respiratory metabolism: increased rate
- repressible
- reduction of function
- conditional
- actin cytoskeleton morphology: abnormal
- cell cycle progression in late anaphase: arrested
- cell cycle progression in telophase: arrested
- cell cycle progression: arrested
- cellular morphology: abnormal
- chromosome segregation: absent
- chromosome/plasmid maintenance: abnormal
- heat sensitivity: increased
- nuclear morphology: abnormal
- protein/peptide accumulation: decreased
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- protein/peptide modification: decreased
- sporulation: absent
- sporulation: decreased
- null
- overexpression
- reduction of function
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
463 total interactions for 222 unique genes
Physical Interactions
- Affinity Capture-MS: 66
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 49
- Biochemical Activity: 57
- Co-crystal Structure: 2
- Co-localization: 7
- Co-purification: 3
- PCA: 1
- Protein-peptide: 2
- Proximity Label-MS: 21
- Reconstituted Complex: 14
- Two-hybrid: 28
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 16
- Dosage Rescue: 41
- Negative Genetic: 15
- Phenotypic Enhancement: 19
- Phenotypic Suppression: 36
- Positive Genetic: 9
- Synthetic Growth Defect: 14
- Synthetic Lethality: 20
- Synthetic Rescue: 37
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2024-11-12
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.