CSM3 / YMR048W Overview
- Standard Name
- CSM3 1
- Systematic Name
- YMR048W
- SGD ID
- SGD:S000004651
- Feature Type
- ORF , Verified
- Description
- Subunit of a replication-pausing checkpoint complex; the replication fork protection complex (Tof1p-Mrc1p-Csm3p) acts at stalled replication forks and is required for rapid replication fork progression, replisome stabilization under stress, mediating DNA replication checkpoint signaling and promoting sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress 1 2 3 4 5
- Name Description
- Chromosome Segregation in Meiosis 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 317
- Mol. Weight (Da)
- 36333.9
- Isoelectric Point
- 4.53
- Median Abundance (molecules/cell)
- 1105 +/- 711
- Half-life (hr)
- 4.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all CSM3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Subunit of the replication fork protection complex that stabilizes stalled replication forks during DNA replication; required for sister chromatid cohesion and chromosome segregation during meiosis; localized to the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables molecular function unknown (ND)
Biological Process
- Manually Curated
- involved in DNA repair (IGI, IMP)
- involved in establishment of mitotic sister chromatid cohesion (IMP, IGI)
- involved in maintenance of DNA repeat elements (IMP)
- involved in meiotic chromosome segregation (IMP)
- involved in mitotic sister chromatid cohesion (IGI, IMP)
- acts upstream of or within replication fork arrest (IMP)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant displays high rate of chromosome instability, increased sensitivity to benomyl, hydroxyurea, camptothecin and selenomethionine; null mutant is also sensitive to UV; in systematic studies mutants are resistant to caffeine, cycloheximide and fluconazole
Classical Genetics
- null
- null
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- competitive fitness: decreased
- haploproficient
- meiosis: abnormal
- mutation frequency: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- spore germination: decreased
- transposable element transposition: increased
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
791 total interactions for 339 unique genes
Physical Interactions
- Affinity Capture-MS: 29
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 45
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 1
- Reconstituted Complex: 14
- Two-hybrid: 8
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 3
- Negative Genetic: 385
- Phenotypic Enhancement: 16
- Phenotypic Suppression: 8
- Positive Genetic: 34
- Synthetic Growth Defect: 171
- Synthetic Lethality: 61
- Synthetic Rescue: 9
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.