Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
12 entries for 10 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| chemical compound accumulation: abnormal | systematic mutation set | null Allele: hot1-Δ | S288C | alpha-amino acid | Details: Significantly altered free amino acid profile (X^2- test adjusted p < 0.01), showing 2 simultaneous amino acid changes (Z-test, adjusted p < 0.01) | Mülleder M, et al. (2016) PMID:27693354 |
| chemical compound accumulation: decreased | systematic mutation set | null Allele: hot1-Δ | S288C | N(2)-acetyl-L-ornithine | Cooper SJ, et al. (2010) PMID:20610602 | |
| chemical compound accumulation: increased | systematic mutation set | null Allele: hot1-Δ | S288C | alanine | Mülleder M, et al. (2016) PMID:27693354 | |
| chemical compound accumulation: increased | systematic mutation set | null Allele: hot1-Δ | S288C | threonine | Mülleder M, et al. (2016) PMID:27693354 | |
| competitive fitness: decreased | competitive growth fitness profiling using complete deletion alleles | null Allele: hot1-Δ | S288C | Media: minimal medium Details: Relative fitness score: 0.922 | Breslow DK, et al. (2008) PMID:18622397 | |
| killer toxin resistance: increased | homozygous diploid | null Allele: hot1-Δ | S288C | Treatment: HM-1 | Miyamoto M, et al. (2012) PMID:23065846 | |
| metal resistance: increased | systematic mutation set | null Allele: hot1-Δ | S288C | lithium(1+) | Treatment: lithium chloride, 200 mM Details: null mutants exhibiting growth below 110% at 200 mM LiCl were classified as having a low level of resistance; screen consisted of a 4 step approach including qualitative assessment on Li-agar plates, liquid microplate assessment, tertiary screening in liquid and a quaternary screen using Li2SO4, followed by classification based on the degree of sensitivity | Fierling N, et al. (2024) PMID:38142127 |
| resistance to chemicals: decreased | systematic mutation set | null Allele: hot1-Δ | S288C | 1 uM myriocin | Fröhlich F, et al. (2015) PMID:26357016 | |
| sporulation: normal | homozygous diploid, systematic mutation set Visual inspection by light microscopy | null Allele: hot1-Δ | SK1 | Rabitsch KP, et al. (2001) PMID:11470404 | ||
| vacuolar morphology: abnormal | systematic mutation set screen all non-essential genes for inability to fragment vacuoles in response to salt addition | null Allele: hot1-Δ | S288C | 0.4 M sodium chloride | Details: small defect in vacuolar fragmentation | Michaillat L and Mayer A (2013) PMID:23383298 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
Addgene Plasmids | DNASU Plasmids | PlasmID | Thermo Scientific | YGRC
dHITS | FitDB | HIPHOP Chemogenomics | HIP HOP Profiles | MetaboGeneCard | PROPHECY | SCMD | ScreenTroll | TheCellVision | Yeast Phenome