Protein Help

TAF7 / YMR227C Protein

Protein abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical properties, protein modification sites, and external identifiers for the protein.


Aliases
TAF67 20 , TafII67
Protein Product
TATA-binding protein-associated factor TAF7
Feature Type
ORF , Verified
Summary
Taf7p is 590 amino acids long, low in abundance, somewhat shorter-lived; sumoylated on 3 lysines, phosphorylated on 8 residues

AlphaFold Protein Structure

AlphaFold, developed by DeepMind, is an AI program that accurately predicts protein structures from amino acid sequences, enabling visualization of protein conformations. The predicted structures can be accessed through the Protein Data Bank (PDB) and AlphaFold Protein Structure Database.


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Model Confidence

Very high
Confident
Low
Very low

Experimental Data

Contains experimentally-derived protein half-life data obtained using stable isotope labeling by amino acids (SILAC) coupled with mass spectrometry. This section also contains protein abundance data for both untreated and treated cells obtained from over 20 studies. These data have been normalized and converted to a common unit of molecules per cell.


Protein Half Life

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ExperimentResultReference
half-life6.8 hrChristiano R, et al. (2014)

Protein Abundance

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Abundance (molecules/cell)MediaTreatmentTreatment timeFold ChangeVisualizationStrain backgroundOriginal ReferenceReference
1703SDuntreatedconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
1579SD200 ng/ml sirolimus700 minconfocal microscopy evidenceS288CChong YT, et al. (2015)Ho B, et al. (2018)
1908SDuntreatedquantitative mass spectrometry evidenceS288Cde Godoy LM, et al. (2008)Ho B, et al. (2018)
1050YEPDuntreatedqualitative western immunoblotting evidenceS288CGhaemmaghami S, et al. (2003)Ho B, et al. (2018)
345YEPDuntreatedquantitative mass spectrometry evidenceS288CKulak NA, et al. (2014)Ho B, et al. (2018)
Showing 1 to 5 of 9 entries

Domains and Classification - S288C

Collection of computationally identified domains and motifs, as determined by InterProScan analysis; includes protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain.


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Gene Protein Coordinates Accession ID Description Source No. of Genes with Domain

Domain Locations

Visual representation of the locations of the domains within the protein, as listed in the Domains and Classification table. Each row displays the domain(s) derived from a different Source, with domains color-coded according to this Source.

Scroll over a domain to view its exact coordinates and its Description.

Shared Domains

This diagram displays domains (colored squares) shared between the given protein (yellow circle) and other proteins (gray circles); the domains are color-coded according to their source, as displayed in the Domain Locations table, above.

Reset

Click on a gene or domain name to go to its specific page within SGD; drag any of the gene or domain objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram.

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.


taf7-N147D | taf7-N147G | taf7-ts1 | taf7-Δ

View all TAF7 alleles in SGD search

Sequence

Protein sequence for the given gene in S288C and other strains, when available. Use the pull-down menu under "Strain" to select the sequence for a specific strain. The displayed sequence can be downloaded in FASTA format as a .txt file. Amino acids displayed in blue represent modification sites. More detailed evidence for these modification sites is presented in the Post-translational Modifications table, located just below the protein sequence.


1 MAVIRIKKPR GPGEKDQPLE GEPKLKRIRI KTKVTDEDIK PKPKLKINLK KKKESADGKE
61 KKNSLKLKLN LKKNEEPVKK IHKAPKLRLK PIRIPGEAYD SEASDIEDDP LIESGVILRI
121 LPDIQLEFVK NSLESGDYSG ISIKWKNERH AVVTINDVMY GAILVDLPTV IEVNKSVDRK
181 NLLKTFDVSQ MLLCIRPIQE EEEVYALEAP DTEDLVVKHF EGIEDEIWEN KETFLKGYNG
241 APLSDMEAKH LKEIALKGYD YKHGISPPLY NVRNRRFRRK MDPNEIDYVE KVVDMLLKQD
301 KQAEEVSYDL VDKSELQARQ ERVSSWENFK EEPGEPLSRP ALKKEEIHTI ASAVGKQGAE
361 EEGEEGMEEE EEEDLDLGAA FESEEEGSGA EGDKEQQQEE VGDEVDQDTG GEDDDDDDDG
421 DIEAAGGESE SDDEKDENRQ HTELLADELN ELETTLAHTK HKLSKATNPL LKSRFIDSIK
481 KLEKEAELKR KQLQQTEDSV QKQHQHRSDA ETANNVEEEE EEEEEEEEED EVDEDEEDDE
541 ENDEDEDNVH EREHIQENKV VRELDEAPAE ETLDQNDLDM MMLFGAEGDE *

* Blue amino acids indicate modification sites. More information below.

Post-translational Modifications - S288C

Modification sites for the protein in the selected strain, based on the presence of a residue in the specific strain, as inferred from experimental evidence.

23 entries for 11 sites

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SiteModificationModifierReference
K40sumoylated lysineBhagwat NR, et al. (2021) PMID: 33502312
Y99phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
Y99phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
S101phosphorylated residueAlbuquerque CP, et al. (2008) PMID: 18407956
S101phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
S101phosphorylated residueHolt LJ, et al. (2009) PMID: 19779198
S101phosphorylated residueZhou X, et al. (2021) PMID: 33481703
S101phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S104phosphorylated residueLanz MC, et al. (2021) PMID: 33491328
S104phosphorylated residueSwaney DL, et al. (2013) PMID: 23749301
Showing 1 to 10 of 23 entries

Sequence-Based Physico-chemical Properties - S288C

Calculated protein properties, including amino acid composition, length, coding region calculations, and atomic composition.

Amino Acid Composition

Sort table using the arrow to the right of a column header to sort by that column; download all properties as a .txt file using the "Download Properties" button.

Amino AcidFrequencyPercentage
A325.42
C10.17
D5910.00
E10617.97
F91.53
G315.25
H132.20
I325.42
K6210.51
L518.64
M101.69
N244.07
P244.07
Q223.73
R244.07
S284.75
T172.88
V325.42
W30.51
Y101.69

Physical Details

Length (a.a): 590
Molecular Weight (Da): 67476.5
Isoelectric Point (pl): 4.21
Formula: C2911H4580N799O1022S11
Aliphatic Index: 71.29
Instability Index: 54.97

Coding Region Translation Calculations

Codon Bias: 0.02
Codon Adaptation Index: 0.14
Frequence of Optimal Codons: 0.45
Hydropathicity of Protein: -1.11
Aromaticity Score: 0.04

Extinction Coefficients at 280nm

ALL Cys residues appear as half cystines: 31400.0
NO Cys residues appear as half cystines: 31400.0

Atomic Composition

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Atom Frequency Percentage

Data not found or not available for  S288C

External Identifiers

List of external identifiers for the protein from various database sources.

18 entries for 8 sources


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External IDSource
orf19.1574CGD
6818DIP
6323883GenBank/EMBL/DDBJ
CAA90198.1GenBank/EMBL/DDBJ
Z49939GenBank/EMBL/DDBJ
2497195GenBank/EMBL/DDBJ
887608GenBank/EMBL/DDBJ
659835411GenBank/EMBL/DDBJ
659835413GenBank/EMBL/DDBJ
659835412GenBank/EMBL/DDBJ
Showing 1 to 10 of 18 entries

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