COA6 / YMR244C-A Overview
- Standard Name
- COA6 1
- Systematic Name
- YMR244C-A
- SGD ID
- SGD:S000004857
- Feature Type
- ORF , Verified
- Description
- Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress 1 2 3 4
- Name Description
- Cytochrome Oxidase Assembly 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Protein abundance increases in response to DNA replication stress
- Length (a.a.)
- 104
- Mol. Weight (Da)
- 12450.3
- Isoelectric Point
- 9.34
- Median Abundance (molecules/cell)
- 3202 +/- 533
- Half-life (hr)
- 5.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all COA6 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Copper-binding protein involved in mitochondrial cytochrome c oxidase assembly; localizes to mitochondrial intermembrane space, nucleus, and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables copper ion binding (IDA)
Biological Process
- Manually Curated
- involved in intracellular copper ion homeostasis (IMP)
- involved in mitochondrial cytochrome c oxidase assembly (IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (HDA)
- located in mitochondrial intermembrane space (IDA)
- located in mitochondrion (HDA)
- located in nucleus (HDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is incapable of respiratory growth on nonfermentable carbon sources and also shows slow vegetative growth on fermentable carbon sources (petite phenotype); in systematic studies null mutant is sensitive to streptomycin, valinomycin and miconazole
Classical Genetics
- null
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- haploproficient
- prion formation: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- toxin resistance: increased
- viable
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast COA6 is homologous to human COA6, and has been used to study mitochondrial cardiomyopathies
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
102 total interactions for 77 unique genes
Physical Interactions
- Affinity Capture-MS: 3
- Affinity Capture-RNA: 9
- Affinity Capture-Western: 5
- Co-localization: 1
- Co-purification: 1
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Rescue: 4
- Negative Genetic: 62
- Phenotypic Enhancement: 2
- Positive Genetic: 10
- Synthetic Growth Defect: 3
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 6
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.