Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.
A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.
24 entries for 16 phenotypesIncrease the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.
| Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details | Reference |
|---|---|---|---|---|---|---|
| chromosome/plasmid maintenance: decreased | classical genetics | null Allele: hhf2-Δ | S288C | Details: 8-fold increase in chromosome instability (CIN) relative to wt using a single-cell quantitative chromosome transmission fidelity (qCTF) assay that detects the loss of an ectopic chromosomal fragment of chrIII; CIN rate is suppressed by 59% when RRM3 is deleted | Gordon MR, et al. (2023) PMID:36350688 | |
| chronological lifespan: increased | systematic mutation set | null Allele: hhf2-Δ | S288C | Campos SE, et al. (2018) PMID:29575540 | ||
| colony sectoring: increased | homozygous diploid, large-scale survey | overexpression | S288C | Details: sectoring due to loss of a chromosome fragment, an indicator of altered chromosome stability | Ouspenski II, et al. (1999) PMID:10454593 | |
| competitive fitness: decreased | competitive growth fitness profiling using complete deletion alleles | null Allele: hhf2-Δ | S288C | Media: minimal medium Details: Relative fitness score: 0.972 | Breslow DK, et al. (2008) PMID:18622397 | |
| haploinsufficient | heterozygous diploid, competitive growth genome-wide fitness profiling | null Allele: hhf2-Δ | S288C | Media: turbidostat growth in FPM medium Details: Relative growth score: -0.0028 | Pir P, et al. (2012) PMID:22244311 | |
| protein activity: increased Reporter: metal reductase | systematic mutation set cell surface metal reductase activity quantified using BPS and scoring colonies for red color | null Allele: hhf2-Δ | S288C | Dong K, et al. (2013) PMID:23959798 | ||
| protein/peptide accumulation: increased Reporter: Cse4p | classical genetics | overexpression | S288C | Details: increased gene dosage of Hhf2p results in an increase in the accumulation of wt Cse4p, and also Cse4-Y1293A, a histone fold domain mutant | Ohkuni K, et al. (2024) PMID:38038247 | |
| protein/peptide modification: decreased Reporter: H3 K79me1 | classical genetics | reduction of function Allele: hhf2-(H4ΔRHRK) basic patch residues of N-terminal tail | Other | Details: H3K79 monomethylation greatly reduced | Fingerman IM, et al. (2007) PMID:17675446 | |
| protein/peptide modification: decreased Reporter: H3 K79me2 | classical genetics | reduction of function Allele: hhf2-(H4Δ4-19) N-terminal tail deletion | Other | Fingerman IM, et al. (2007) PMID:17675446 | ||
| protein/peptide modification: increased Reporter: Cse4p | classical genetics | overexpression | S288C | Details: increased gene dosage of Hhf2p results in a significant increase in the sumoylation of wt Cse4p, and also Cse4-Y1293A, a histone fold domain mutant | Ohkuni K, et al. (2024) PMID:38038247 |
This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).
Click on a gene or phenotype observable name to go to its specific page within SGD; drag any of the gene or observable objects around within the visualization for easier viewing; click “Reset” to automatically redraw the diagram; filter the genes that share observable terms with the given gene by the number of terms they share by clicking anywhere on the slider bar or dragging the tab to the desired filter number.
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