SIW14 / YNL032W Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided.

Summary

SIW14/YNL032W is a non-essential gene in strains S288C; null mutants are viable but show impaired growth in inhibitor-stressed media (5‑hydroxymethylfurfural, acetic acid, furfural) and altered cell biology, including abnormal vacuoles (high salt), endoplasmic reticulum, and actin cytoskeleton, reduced endocytosis, abnormal protein distribution, shorter telomeres, and decreased transposable element transposition. Respiration is defective (no growth on glycerol). Stress responses are mixed: heat tolerance is improved (decreased heat sensitivity and increased thermotolerance), hyperosmotic resistance is elevated (KCl), oxidative stress tolerance is higher to H2O2 but lower to paraquat, and both replicative and chronological lifespans are increased. Drug and toxin sensitivities are broad, with decreased resistance to many agents (caffeine, tunicamycin, cycloheximide, hydroxyurea, MMS, bleomycin, fluconazole, cordycepin, camptothecin, hygromycin B, DTT, octanoic/tannic acids, toxaphene, vanillin, 4‑MCHM, high HMF), yet increased resistance to canavanine and several metals/salts (Mn2+, Ni2+, Co2+, Gd3+, Y3+) and decreased resistance to Hg2+. Toxin responses are context‑dependent (general increase but reduced resistance to Aβ42), ROS accumulate under HMF, competitive fitness shifts with medium (higher in YPD/OAK, lower in minimal, SC, or ethanol‑rich), pseudohyphal growth increases under low nitrogen with RNA/IP polyphosphate changes (PP‑IP4/PP‑IP5 up, IP6 down), and threonine levels decrease alongside a broader amino acid imbalance.

Annotations

A phenotype is defined as an observable (e.g., apoptosis) and a qualifier (e.g., increased). There may be more than one row with the same phenotype if that phenotype was observed in separate studies or in different conditions, strains, alleles, etc.

Shared Phenotypes

This diagram displays phenotype observables (purple squares) that are shared between the given gene (yellow circle) and other genes (gray circles) based on the number of phenotype observables shared (adjustable using the slider at the bottom).

Resources