YDJ1 / YNL064C Overview
- Standard Name
- YDJ1 1
- Systematic Name
- YNL064C
- SGD ID
- SGD:S000005008
- Aliases
- MAS5 32 , HSP40 , MAB3 33
- Feature Type
- ORF , Verified
- Description
- Type I HSP40 co-chaperone; regulates HSP90 and HSP70 functions; acts as an adaptor that helps Rsp5p recognize and ubiquitinate misfolded, cytosolic proteins after heat shock; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; forms cytotoxic amyloidogenic-like structures in vitro; DnaJ family member; chimeric protein in which the human p58IPK J domain replaces the yeast Ydj1p J domain can complement yeast ydj1 mutant 1 2 3 4 5 6 7 8 9 10
- Name Description
- Yeast dnaJ 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Ydj1p is 409 amino acids long; acetylated on 12 residues; N-terminal acetylation of the J domain fine-tunes translational fidelity and client protein refolding
- Length (a.a.)
- 409
- Mol. Weight (Da)
- 44666.2
- Isoelectric Point
- 6.21
- Median Abundance (molecules/cell)
- 41251 +/- 16052
- Half-life (hr)
- 11.4
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all YDJ1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- ATPase-activating protein chaperone involved in 'de novo' protein folding, refolding and catabolism; subunit of the TMD recognition ER membrane insertion complex; localizes to the cytoplasm near the nucleus
View computational annotations
Molecular Function
- Manually Curated
- enables ATPase activator activity (IDA)
- enables unfolded protein binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in 'de novo' protein folding (IMP)
- involved in cellular response to heat (IMP)
- involved in cellular response to starvation (IMP)
- involved in ERAD pathway (IMP)
- involved in protein refolding (IDA)
- involved in protein targeting to ER (IMP)
- involved in protein targeting to mitochondrion (IMP)
- involved in tRNA import into nucleus (IMP)
- involved in ubiquitin-dependent protein catabolic process (IMP)
Cellular Component
- Manually Curated
- located in cytosol (IDA, HDA)
- located in perinuclear region of cytoplasm (IDA)
- part of TRC complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant exhibits slow growth, decreased fitness and lifespan, abnormal colony shape and vacuolar morphology, increased sensitivity to heat, oxidative stress, metals, caffeine, cardivascular drug amiodarone, phosphatidylinositol 3-kinase inhibitor wortmannin, Hsp90 inhibitor tanespimycin, TOR inhibitor rapamycin, antioxidant spermine, reducing agent L-1,4-dithiothreitol, microtubule-destabilising agent benomyl, protein synthesis inbibitor cycloheximide, herbicide amitrole, allergen glyoxal, and various alcohols, antibiotics, antifungals, and mutagens; homozygous diploid null mutant displays increased resistance to carcinogen benzo[a]pyrene, and is sensitive to zinc, sorbate, mycotoxin Trichothecin; overexpression confers resistance to TOR inhibitor rapamycin and causes increased invasive growth and prion loss
Classical Genetics
- null
- colony shape: abnormal
- gamma ray resistance: decreased
- heat sensitivity: increased
- innate thermotolerance: decreased
- mating efficiency: decreased
- metal resistance: decreased
- oxidative stress resistance: decreased
- prion loss: absent
- prion loss: increased
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- stress resistance: decreased
- toxin resistance: decreased
- UV resistance: decreased
- vegetative growth: decreased
- overexpression
- reduction of function
- conditional
- unspecified
- null
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- cold sensitivity: increased
- competitive fitness: decreased
- heat sensitivity: increased
- hydrostatic pressure resistance: decreased
- metal resistance: decreased
- mutation frequency: increased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- peroxisomal morphology: abnormal
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- respiratory growth: absent
- respiratory growth: decreased rate
- stress resistance: decreased
- stress resistance: increased
- toxin resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- unspecified
- overexpression
- conditional
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast YDJ1 is homologous to human DNAJA1, and has been used to study amyloid beta 42 toxicity as the principal trigger of neurodegeneration during Alzheimer's disease (AD); DNAJA1 has been found to be dysregulated in post mortem brains of AD patients
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
1457 total interactions for 979 unique genes
Physical Interactions
- Affinity Capture-MS: 157
- Affinity Capture-RNA: 10
- Affinity Capture-Western: 39
- Biochemical Activity: 7
- Co-crystal Structure: 2
- Co-fractionation: 1
- Co-localization: 1
- Co-purification: 4
- Cross-Linking-MS (XL-MS): 2
- PCA: 1
- Proximity Label-MS: 3
- Reconstituted Complex: 26
- Two-hybrid: 1
Genetic Interactions
- Dosage Growth Defect: 8
- Dosage Rescue: 20
- Negative Genetic: 886
- Phenotypic Enhancement: 8
- Phenotypic Suppression: 5
- Positive Genetic: 197
- Synthetic Growth Defect: 28
- Synthetic Lethality: 44
- Synthetic Rescue: 7
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 10
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-12-19
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.