WHI3 / YNL197C Overview
- Standard Name
- WHI3 1
- Systematic Name
- YNL197C
- SGD ID
- SGD:S000005141
- Feature Type
- ORF , Verified
- Description
- RNA binding protein that modulates mRNA stability; regulates cell cycle, sister chromatid cohesion, and stress response genes; modulates stability and translational efficiency of CLN3 mRNA; colocalizes with P-bodies/stress granules to regulate target mRNAs; regulates cell fate and critical cell size for Start passage; regulates ploidy; self-templating mnemon that forms inactive super-assemblies, preventing G1 arrest in mother cells after unsuccessful mating and causing sterility in old cells 1 2 3 4 5 6 7 8 9 10
- Name Description
- WHIskey 11
- Paralog
- WHI4 10
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 661
- Mol. Weight (Da)
- 71257.3
- Isoelectric Point
- 8.59
- Median Abundance (molecules/cell)
- 5072 +/- 835
- Half-life (hr)
- 8.6
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all WHI3 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Cytoplasmic mRNA-binding protein involved in mRNA catabolism, invasive growth, and traversing the start control point of the mitotic cell cycle; locallizes to stress granules and P-bodies
View computational annotations
Molecular Function
- Manually Curated
- enables mRNA binding (IDA, IMP)
Biological Process
- Manually Curated
- involved in invasive growth in response to glucose limitation (IMP)
- involved in mRNA destabilization (IMP)
- involved in pseudohyphal growth (IMP)
- involved in regulation of cell size (HMP)
- involved in regulation of mRNA catabolic process (IMP)
- involved in traversing start control point of mitotic cell cycle (IGI, IMP)
Cellular Component
- Manually Curated
- located in cytoplasm (IDA)
- located in cytoplasmic stress granule (IDA, HDA)
- located in endoplasmic reticulum membrane (IDA)
- located in P-body (IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant has a decreased critical cell size at START, while overexpression results in G1 arrest as large unbudded cells with decreased viability; null mutant has an invasive growth defect, decreased mating efficiency and pheromone sensitivity; null mutant is sensitive to translation elongation inhibitors, and spontaneously diploidizes due to an increased rate of whole-genome duplication; homozygous diploid null has a pseudohyphal growth defect, decreased sporulation efficiency, decreased chronological lifespan and decreased desiccation resistance, while the heterozygous null is haploinsufficient
Classical Genetics
- null
- cell death: absent
- cell shape: abnormal
- cell size: decreased
- critical cell size at START (G1 cell-size checkpoint): decreased
- growth in exponential phase: normal rate
- invasive growth: decreased
- mating efficiency: decreased
- metal resistance: decreased
- pheromone sensitivity: decreased
- polyploidy: increased rate
- protein/peptide distribution: abnormal
- pseudohyphal growth: decreased
- resistance to chemicals: decreased
- sporulation efficiency: decreased
- viable
- overexpression
- unspecified
- reduction of function
- null
- cell size: decreased
- chemical compound accumulation: abnormal
- chemical compound accumulation: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- desiccation resistance: decreased
- endocytosis: decreased
- haploinsufficient
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- liquid culture appearance: abnormal
- oxidative stress resistance: increased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- resistance to chemicals: normal
- sporulation efficiency: decreased
- vacuolar morphology: abnormal
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The whi3 null mutant is viable; the null mutant of paralog whi4 is viable; the whi3 whi4 double mutant displays negative genetic interactions.
723 total interactions for 556 unique genes
Physical Interactions
- Affinity Capture-MS: 19
- Affinity Capture-RNA: 113
- Affinity Capture-Western: 7
- Biochemical Activity: 4
- Co-localization: 1
- Co-purification: 1
- PCA: 13
- Protein-RNA: 317
- Proximity Label-MS: 1
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 149
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 7
- Positive Genetic: 61
- Synthetic Growth Defect: 12
- Synthetic Lethality: 7
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Summary Paragraph
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.
Last Updated: 2006-10-27
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.