ALR1 / YOL130W Overview
- Standard Name
- ALR1 1
- Systematic Name
- YOL130W
- SGD ID
- SGD:S000005490
- Aliases
- SWC3 5
- Feature Type
- ORF , Verified
- Description
- Plasma membrane Mg(2+) transporter; expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; magnesium transport defect of the null mutant is functionally complemented by either of the human genes MAGT1 and TUSC3 that are not orthologous to ALR1 1 2 3 4
- Name Description
- ALuminum Resistance 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 859
- Mol. Weight (Da)
- 95860.4
- Isoelectric Point
- 6.68
- Median Abundance (molecules/cell)
- 1312 +/- 648
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all ALR1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Magnesium transmembrane transporter; localized in plasma membrane
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in magnesium ion transport (IDA)
- involved in monoatomic cation transport (IMP)
Cellular Component
- Manually Curated
- located in cell periphery (HDA)
- located in plasma membrane (IDA, IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Null mutants grow very slowly; reduced function mutants show decreased competitive fitness, increased sensitivity to microtubule-destabilizing agent benomyl; overexpression slows growth, increases sensitivity to rapamycin
Classical Genetics
- null
- overexpression
- reduction of function
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast ALR1 is homologous to human MAGT1 and TUSC3, and has been used to study primary hypomagnesemia
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
61 total interactions for 50 unique genes
Physical Interactions
- Affinity Capture-MS: 7
- Affinity Capture-RNA: 6
- Biochemical Activity: 6
- Co-purification: 1
- PCA: 3
- Two-hybrid: 6
Genetic Interactions
- Dosage Lethality: 2
- Dosage Rescue: 1
- Negative Genetic: 16
- Phenotypic Enhancement: 2
- Phenotypic Suppression: 1
- Synthetic Growth Defect: 2
- Synthetic Lethality: 2
- Synthetic Rescue: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- ALR1 encodes a plasma membrane magnesium transporter that is primarily responsible for Mg2+ uptake. Additionally, Alr1p can act as a low-affinity transporter of other divalent cations such as Co2+, Mn2+, Ni2+ and Zn2+. Yeast cells also have another Mg2+ transporter Alr2p, which is closely related to Alr1p but appears to play a minor role in Mg2+ uptake. Intracellular levels of Mg2+ are critically important since Mg2+ is a cofactor for hundreds of enzymes and, after potassium, the second most abundant cation in the cell. In addition to uptake, cytoplasmic concentration of Mg2+ is also affected by sequestration in the vacuole that is controlled by a vacuolar membrane transporter Mnr2p. Consistent with its key role in Mg2+ homeostasis, Alr1p activity is regulated by Mg2+ availability, but the exact mechanisms of this regulation remain unclear. There are conflicting reports regarding the transcriptional regulation of ALR1. In one report, the transcription of ALR1 gene is shown to respond to extracellular Mg2+. In another report, ALR1 is only slightly induced by low levels of Mg2+, suggesting that transcriptional regulation does not play a significant role. The plasma membrane localization and turnover of Alr1p are also potential targets of magnesium-dependent regulation, and Alr1p has been shown to be rapidly degraded under high Mg2+ concentrations via ubiquitination, endocytosis, and vacuolar degradation. However, a later report suggests that this could have been an artifact of an HA-tagged protein since the native Alr1p does not appear to be a subject of such regulation. Thus, the mechanism of magnesium-dependent regulation of Alr1p remains to be elucidated.
- Regulators
- 10
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.