HRT1 / YOL133W Overview
- Standard Name
- HRT1 1
- Systematic Name
- YOL133W
- SGD ID
- SGD:S000005493
- Aliases
- ROC1 5 , RBX1 , HRT2
- Feature Type
- ORF , Verified
- Description
- RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair 1 2 3 4 5 6 7 8
- Name Description
- High level expression Reduces Ty3 transposition 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 121
- Mol. Weight (Da)
- 13936.0
- Isoelectric Point
- 5.6
- Median Abundance (molecules/cell)
- 7964 +/- 6251
- Half-life (hr)
- 6.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all HRT1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- SCF, Cul3-RING and Cul8-RING ubiquitin ligase complex subunit involved in G1/S transition of mitotic cell cycle; contributes to protein ubiquitination involved in ubiquitin-dependent protein degradation, SCF-dependent proteasomal ubiquitin-dependent protein degradation; localizes to nucleus and cytoplasm
View computational annotations
Molecular Function
- Manually Curated
- enables protein-macromolecule adaptor activity (IDA)
- contributes to ubiquitin-protein transferase activity (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in G1/S transition of mitotic cell cycle (IMP)
- involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process (IDA)
- involved in ubiquitin-dependent protein catabolic process (IDA, IMP)
Cellular Component
- Manually Curated
- part of Cul3-RING ubiquitin ligase complex (IDA)
- part of Cul8-RING ubiquitin ligase complex (IDA)
- located in cytoplasm (IDA)
- located in nucleus (IDA)
- part of SCF ubiquitin ligase complex (IPI, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
- CUL3-HRT1-ELC1-ELA1 ubiquitin ligase complex
- CUL8-MMS1-ESC2 E3 ubiquitin ligase complex
- CUL8-MMS1-MMS22-CTF4 E3 ubiquitin ligase complex
- CUL8-MMS1-MMS22-ESC4 E3 ubiquitin ligase complex
- CUL8-MMS1-ORC5 E3 ubiquitin ligase complex
- SCF-Cdc4 ubiquitin ligase complex
- SCF-Das1 ubiquitin ligase complex
- SCF-Dia2 ubiquitin ligase complex
- SCF-Grr1 ubiquitin ligase complex
- SCF-Hrt3 ubiquitin ligase complex
- SCF-MET30 E3 ubiquitin ligase complex
- SCF-Mdm30 ubiquitin ligase complex
- SCF-Saf1 ubiquitin ligase complex
- SCF-Ufo1 ubiquitin ligase complex
- SCF-Ydr131c ubiquitin ligase complex
- SCF-Ylr352w ubiquitin ligase complex
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Essential gene; reduction-of-function mutations cause decreased telomere length and increased sensitivity to MMS, sulfanilamide and verrucarin A
Classical Genetics
- null
- null
- reduction of function
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
171 total interactions for 99 unique genes
Physical Interactions
- Affinity Capture-MS: 47
- Affinity Capture-RNA: 8
- Affinity Capture-Western: 22
- Biochemical Activity: 1
- Co-crystal Structure: 1
- Co-purification: 6
- FRET: 1
- Reconstituted Complex: 47
- Two-hybrid: 14
Genetic Interactions
- Dosage Growth Defect: 1
- Dosage Lethality: 5
- Dosage Rescue: 1
- Negative Genetic: 4
- Synthetic Growth Defect: 3
- Synthetic Lethality: 10
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 3
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.