RTS1 / YOR014W Overview
- Standard Name
- RTS1 1
- Systematic Name
- YOR014W
- SGD ID
- SGD:S000005540
- Aliases
- SCS1 6
- Feature Type
- ORF , Verified
- Description
- B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A 1 2 3 4 5
- Name Description
- Rox Three Suppressor 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 757
- Mol. Weight (Da)
- 85323.6
- Isoelectric Point
- 6.25
- Median Abundance (molecules/cell)
- 4713 +/- 905
- Half-life (hr)
- 8.8
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all RTS1 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein phosphatase regulator involved in protein dephosphorylation, mitotic spindle orientation checkpoint, and septin ring organization and disassembly; also involved in sister chromatid biorientation, meiotic sister chromatid cohesion at centromeres, and establishment of protein localization to chromosome; localizes to the nucleus, kinetochore, spindle pole body, bud neck, and centromeric regions of chromosomes
View computational annotations
Molecular Function
- Manually Curated
- enables DNA binding (IDA)
- enables protein phosphatase regulator activity (IDA)
Biological Process
- Manually Curated
- involved in cellular bud neck septin ring organization (IMP, IGI)
- involved in DNA repair (IMP)
- involved in establishment of protein localization to chromosome (IMP)
- involved in meiotic sister chromatid cohesion, centromeric (IMP)
- involved in mitotic spindle orientation checkpoint signaling (IGI)
- involved in positive regulation of autophagosome assembly (IGI)
- involved in protein localization (IMP)
- involved in septin ring disassembly (IMP)
- involved in sister chromatid biorientation (IGI)
Cellular Component
- Manually Curated
- located in cellular bud neck (IDA)
- located in cytoplasm (HDA)
- located in kinetochore (IDA)
- located in nucleus (IDA, HDA)
- located in spindle pole body (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant is sensitive to elevated temperatures and accumulates glycogen and glutathione; in systematic studies null mutants show slow growth, lower competitive fitness, decreased chronological lifespan and increased sensitivity to hyperosmotic stress, bleomycin, cycloheximide, MMS, hygromycin B, ethanol, fluconazole, but increased resistance to tunicamycin, mycophenolic acid, acetic acid
Classical Genetics
- null
- acid pH resistance: decreased
- cell size: increased
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chemical compound excretion: increased
- chronological lifespan: decreased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- innate thermotolerance: increased
- mutation frequency: increased
- oxidative stress resistance: decreased
- oxidative stress resistance: increased
- protein/peptide accumulation: decreased
- replicative lifespan: decreased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- toxin resistance: decreased
- utilization of nitrogen source: decreased rate
- vegetative growth: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
- conditional
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
746 total interactions for 468 unique genes
Physical Interactions
- Affinity Capture-MS: 40
- Affinity Capture-RNA: 3
- Affinity Capture-Western: 10
- Biochemical Activity: 2
- Co-localization: 1
- PCA: 1
- Reconstituted Complex: 3
- Two-hybrid: 6
Genetic Interactions
- Dosage Growth Defect: 2
- Dosage Lethality: 1
- Dosage Rescue: 11
- Negative Genetic: 443
- Phenotypic Enhancement: 19
- Phenotypic Suppression: 19
- Positive Genetic: 111
- Synthetic Growth Defect: 33
- Synthetic Haploinsufficiency: 1
- Synthetic Lethality: 18
- Synthetic Rescue: 22
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.