IDH2 / YOR136W Overview
- Standard Name
- IDH2 1
- Systematic Name
- YOR136W
- SGD ID
- SGD:S000005662
- Feature Type
- ORF , Verified
- Description
- Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes oxidation of isocitrate to alpha-ketoglutarate in TCA cycle; targeted to vacuole via AP-3 pathway 1 2 3
- Name Description
- Isocitrate DeHydrogenase 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 369
- Mol. Weight (Da)
- 39743.9
- Isoelectric Point
- 9.01
- Median Abundance (molecules/cell)
- 18982 +/- 8812
- Half-life (hr)
- 15.5
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results.
View all IDH2 alleles in SGD search
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Catalytic subunit of the mitochondrial isocitrate dehydrogenase complex; has roles in isocitrate metabolism, glutamate biosynthesis, and the tricarboxylic acid cycle
View computational annotations
Molecular Function
- Manually Curated
- contributes to isocitrate dehydrogenase (NAD+) activity (IDA)
Biological Process
- Manually Curated
- involved in glutamate biosynthetic process (TAS)
- involved in isocitrate metabolic process (IDA)
- involved in tricarboxylic acid cycle (TAS)
Cellular Component
- Manually Curated
- part of isocitrate dehydrogenase complex (NAD+) (IDA)
- located in mitochondrion (HDA, IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Pathways
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- IDH2/YOR136W is a non-essential gene; null mutants are viable but exhibit a wide range of phenotypic changes including decreased chronological lifespan, increased replicative lifespan, increased innate thermotolerance, decreased nutrient utilization, increased oxidative stress resistance, decreased respiratory growth rate, decreased biofilm formation, increased duration of cell cycle progression in the G1 phase, abnormal chromosome/plasmid maintenance, abnormal colony appearance, altered competitive fitness, increased heat sensitivity, decreased hyperosmotic stress resistance, absent invasive growth, decreased sporulation efficiency, increased stress resistance, decreased toxin resistance, impaired utilization of various carbon and nitrogen sources, abnormal vacuolar morphology, and decreased rate of vegetative growth. Reduction of function mutants show decreased mitochondrial genome maintenance, decreased respiratory growth rate, and decreased rate of vegetative growth. Overexpression of IDH2 results in increased resistance to certain chemicals.
Classical Genetics
- null
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- innate thermotolerance: increased
- nutrient utilization: decreased
- oxidative stress resistance: increased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- utilization of carbon source: absent
- unspecified
- reduction of function
- null
- biofilm formation: decreased
- cell cycle progression in G1 phase: increased duration
- chemical compound accumulation: decreased
- chromosome/plasmid maintenance: abnormal
- chronological lifespan: increased
- colony appearance: abnormal
- competitive fitness: decreased
- competitive fitness: increased
- heat sensitivity: increased
- hyperosmotic stress resistance: decreased
- invasive growth: absent
- oxidative stress resistance: increased
- protein/peptide accumulation: increased
- replicative lifespan: decreased
- replicative lifespan: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased
- respiratory growth: decreased rate
- sporulation efficiency: decreased
- stress resistance: increased
- toxin resistance: decreased
- utilization of carbon source: decreased
- utilization of nitrogen source: decreased rate
- vacuolar morphology: abnormal
- vegetative growth: decreased rate
- viable
- overexpression
Large-scale Survey
Disease
Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.
- Summary
- Yeast IDH2 is homologous to human IDH3A, and has been used to study severe encephalopathy in infancy
Manually Curated
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
526 total interactions for 384 unique genes
Physical Interactions
- Affinity Capture-MS: 133
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 2
- Co-crystal Structure: 2
- Co-localization: 1
- Co-purification: 2
- Cross-Linking-MS (XL-MS): 7
- PCA: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 2
- Two-hybrid: 4
Genetic Interactions
- Dosage Rescue: 2
- Negative Genetic: 261
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 10
- Positive Genetic: 73
- Synthetic Growth Defect: 10
- Synthetic Lethality: 4
- Synthetic Rescue: 2
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 16
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.